Nocardioides basaltis sp. nov., isolated from black beach sand

A novel Gram-positive, aerobic, short-rod-shaped bacterium, designated strain J112^T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. The strain was found to be oxidase-negative and catalase-positive. Cells grew at 10-37 °C, at pH 5.5-8.0 and with 1-10% NaCl. Growth occurred on marine agar but not on R2A or trypticase soy agar. A phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belongs to the radiation of the genus Nocardioides. Strain J112^T shared the highest 16S rRNA gene sequence similarities with Nocardioides marinisabuli SBS-12^T (99.2%), Nocardioides terrigena DS-17^T (97.3%), Nocardioides kribbensis KCTC 19038^T (97.1%) and type strains of other Nocardioides species with validly published names (<97%). The DNA-DNA hybridization values between strain J112^T and the three most closely related strains were low enough to justify the assignment of this strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain J112^T represents a novel species of the genus Nocardioides, for which the name Nocardioides basaltis sp. nov. is proposed. The type strain is J112^T (=KCTC 19365^T=JCM 14945^T).