Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers

Chengfan Cao; Sanghyun Hong; Jae Boong Choi; Young Jin Kim; Seunghyun Baik; Eung Soo Hwang; Hye Jin Jung; Ng Yong Cha

doi:10.1109/ICSENS.2007.355861

Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers

Chengfan Cao, Sanghyun Hong, Jae Boong Choi, Young Jin Kim, Seunghyun Baik, Eung Soo Hwang, Hye Jin Jung, Ng Yong Cha

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.

Original language	English
Title of host publication	2006 5th IEEE Conference on Sensors
Pages	1272-1275
Number of pages	4
DOIs	https://doi.org/10.1109/ICSENS.2007.355861
State	Published - 2006
Event	2006 5th IEEE Conference on Sensors - Daegu, Korea, Republic of Duration: 22 Oct 2006 → 25 Oct 2006

Publication series

Name	Proceedings of IEEE Sensors

Conference

Conference	2006 5th IEEE Conference on Sensors
Country/Territory	Korea, Republic of
City	Daegu
Period	22/10/06 → 25/10/06

Access to Document

10.1109/ICSENS.2007.355861

Cite this

@inproceedings{6a1772b43ca241d38601bffdf56b9fc7,

title = "Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers",

abstract = "Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.",

author = "Chengfan Cao and Sanghyun Hong and Choi, \{Jae Boong\} and Kim, \{Young Jin\} and Seunghyun Baik and Hwang, \{Eung Soo\} and Jung, \{Hye Jin\} and Cha, \{Ng Yong\}",

year = "2006",

doi = "10.1109/ICSENS.2007.355861",

language = "English",

isbn = "1424403766",

series = "Proceedings of IEEE Sensors",

pages = "1272--1275",

booktitle = "2006 5th IEEE Conference on Sensors",

note = "2006 5th IEEE Conference on Sensors ; Conference date: 22-10-2006 Through 25-10-2006",

}

Cao, C, Hong, S, Choi, JB, Kim, YJ, Baik, S, Hwang, ES, Jung, HJ & Cha, NY 2006, Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers. in 2006 5th IEEE Conference on Sensors., 4178856, Proceedings of IEEE Sensors, pp. 1272-1275, 2006 5th IEEE Conference on Sensors, Daegu, Korea, Republic of, 22/10/06. https://doi.org/10.1109/ICSENS.2007.355861

TY - GEN

T1 - Nanotube molecular probes

T2 - 2006 5th IEEE Conference on Sensors

AU - Cao, Chengfan

AU - Hong, Sanghyun

AU - Choi, Jae Boong

AU - Kim, Young Jin

AU - Baik, Seunghyun

AU - Hwang, Eung Soo

AU - Jung, Hye Jin

AU - Cha, Ng Yong

PY - 2006

Y1 - 2006

N2 - Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.

AB - Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.

UR - https://www.scopus.com/pages/publications/50149089616

U2 - 10.1109/ICSENS.2007.355861

DO - 10.1109/ICSENS.2007.355861

M3 - Conference contribution

AN - SCOPUS:50149089616

SN - 1424403766

SN - 9781424403769

T3 - Proceedings of IEEE Sensors

SP - 1272

EP - 1275

BT - 2006 5th IEEE Conference on Sensors

Y2 - 22 October 2006 through 25 October 2006

ER -

Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers

Abstract

Publication series

Conference

Access to Document

Other files and links

Fingerprint

Cite this