Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment

Ji Hoon Kim, So Jung Kang, Yong Taek Jung, Tae Kwang Oh, Jung Hoon Yoon

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36 Scopus citations

Abstract

A Gram-staining-negative, non-spore-forming, facultatively aerobic, non-motile, rod-shaped bacterial strain, BR-3 T, was isolated from a tidal flat on the western coast of Korea, and subjected to a polyphasic study. Strain BR-3 T grew optimally at 25°6C, at pH 6.5-7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-3T fell within the clade comprising species of the genus Mucilaginibacter, joining the type strain of Mucilaginibacter rigui, with which it exhibited highest 16S rRNA gene sequence similarity (98.2%). 16S rRNA gene sequence similarity values between strain BR-3 T and the type strains of the other species of the genus Mucilaginibacter were in the range 93.8-95.9%. A mean DNA-DNA relatedness value between strain BR-3 T and M. rigui KCTC 12534 T was 21%. Strain BR-3 T contained MK-7 as the predominant menaquinone and C 16: 1ω7c and/or iso-C 15: 0 2-OH and iso-C 15: 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The DNA G+C content was 49.8 mol%. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain BR-3T demonstrated that this strain is separate from M. rigui as well as the other species of the genus Mucilaginibacter. On the basis of the data presented, strain BR-3 T is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter lutimaris sp. nov. is proposed. The type strain is BR-3 T (=KCTC 23461 T =CCUG 60742 T).

Original languageEnglish
Pages (from-to)515-519
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume62
Issue numberPART 3
DOIs
StatePublished - Mar 2012

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