Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

Min Kyu Jeong; Ju Dong Yeo; Eun Yeong Jang; Jae Hwan Lee

doi:10.1007/s10068-013-0062-4

Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

Min Kyu Jeong, Ju Dong Yeo, Eun Yeong Jang, Jae Hwan Lee

Department of Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Oxidized lipids can decrease the absorbance of DPPH in isooctane. Changes of aldehydes, which can react with DPPH radicals, in oxidized linoleic acid and lard were monitored using 2,4-dinitrophenylhydrazine (DNPH) derivatization and HPLC. As thermal oxidation time increased in linoleic acid, peaks of propanal, pentanal, and hexanal increased proportionally. The correlation coefficient (r) of among DPPH absorbance and peak areas of DNPH derivatized lard for 360 min was -0. 941. Peaks of t-2-heptenal and pentanal showed relatively high correlation with the changes of DPPH absorbance during lard oxidation. Quantity of oxidized products with radical scavenging ability in lipids can be determined by DNPH derivatization with HPLC analysis.

Original language	English
Pages (from-to)	161-165
Number of pages	5
Journal	Food Science and Biotechnology
Volume	22
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1007/s10068-013-0062-4
State	Published - 2013

Keywords

2,4-dinitrophenylhydrazine (DNPH) derivatization
DPPH absorbance
lard
linoleic acid
radical scavenging compounds from oxidized lipids (RSOL)

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10.1007/s10068-013-0062-4

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title = "Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems",

abstract = "Oxidized lipids can decrease the absorbance of DPPH in isooctane. Changes of aldehydes, which can react with DPPH radicals, in oxidized linoleic acid and lard were monitored using 2,4-dinitrophenylhydrazine (DNPH) derivatization and HPLC. As thermal oxidation time increased in linoleic acid, peaks of propanal, pentanal, and hexanal increased proportionally. The correlation coefficient (r) of among DPPH absorbance and peak areas of DNPH derivatized lard for 360 min was -0. 941. Peaks of t-2-heptenal and pentanal showed relatively high correlation with the changes of DPPH absorbance during lard oxidation. Quantity of oxidized products with radical scavenging ability in lipids can be determined by DNPH derivatization with HPLC analysis.",

keywords = "2,4-dinitrophenylhydrazine (DNPH) derivatization, DPPH absorbance, lard, linoleic acid, radical scavenging compounds from oxidized lipids (RSOL)",

author = "Jeong, \{Min Kyu\} and Yeo, \{Ju Dong\} and Jang, \{Eun Yeong\} and Lee, \{Jae Hwan\}",

year = "2013",

doi = "10.1007/s10068-013-0062-4",

language = "English",

volume = "22",

pages = "161--165",

journal = "Food Science and Biotechnology",

issn = "1226-7708",

publisher = "The Korean Society of Food Science and Technology",

number = "SUPPL. 1",

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Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems. / Jeong, Min Kyu; Yeo, Ju Dong; Jang, Eun Yeong et al.
In: Food Science and Biotechnology, Vol. 22, No. SUPPL. 1, 2013, p. 161-165.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

AU - Jeong, Min Kyu

AU - Yeo, Ju Dong

AU - Jang, Eun Yeong

AU - Lee, Jae Hwan

PY - 2013

Y1 - 2013

N2 - Oxidized lipids can decrease the absorbance of DPPH in isooctane. Changes of aldehydes, which can react with DPPH radicals, in oxidized linoleic acid and lard were monitored using 2,4-dinitrophenylhydrazine (DNPH) derivatization and HPLC. As thermal oxidation time increased in linoleic acid, peaks of propanal, pentanal, and hexanal increased proportionally. The correlation coefficient (r) of among DPPH absorbance and peak areas of DNPH derivatized lard for 360 min was -0. 941. Peaks of t-2-heptenal and pentanal showed relatively high correlation with the changes of DPPH absorbance during lard oxidation. Quantity of oxidized products with radical scavenging ability in lipids can be determined by DNPH derivatization with HPLC analysis.

AB - Oxidized lipids can decrease the absorbance of DPPH in isooctane. Changes of aldehydes, which can react with DPPH radicals, in oxidized linoleic acid and lard were monitored using 2,4-dinitrophenylhydrazine (DNPH) derivatization and HPLC. As thermal oxidation time increased in linoleic acid, peaks of propanal, pentanal, and hexanal increased proportionally. The correlation coefficient (r) of among DPPH absorbance and peak areas of DNPH derivatized lard for 360 min was -0. 941. Peaks of t-2-heptenal and pentanal showed relatively high correlation with the changes of DPPH absorbance during lard oxidation. Quantity of oxidized products with radical scavenging ability in lipids can be determined by DNPH derivatization with HPLC analysis.

KW - 2,4-dinitrophenylhydrazine (DNPH) derivatization

KW - DPPH absorbance

KW - lard

KW - linoleic acid

KW - radical scavenging compounds from oxidized lipids (RSOL)

UR - https://www.scopus.com/pages/publications/84874910403

U2 - 10.1007/s10068-013-0062-4

DO - 10.1007/s10068-013-0062-4

M3 - Article

AN - SCOPUS:84874910403

SN - 1226-7708

VL - 22

SP - 161

EP - 165

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

IS - SUPPL. 1

ER -

Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

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