Moesin (MSN) as a novel proteome-based diagnostic marker for early detection of invasive bladder urothelial carcinoma in liquid-based cytology

Jeong Hwan Park; Cheol Lee; Dohyun Han; Jae Seok Lee; Kyung Min Lee; Min Ji Song; Kwangsoo Kim; Heonyi Lee; Kyung Chul Moon; Youngsoo Kim; Minsun Jung; Ji Hye Moon; Hyebin Lee; Han Suk Ryu

doi:10.3390/cancers12041018

Moesin (MSN) as a novel proteome-based diagnostic marker for early detection of invasive bladder urothelial carcinoma in liquid-based cytology

Jeong Hwan Park, Cheol Lee, Dohyun Han, Jae Seok Lee, Kyung Min Lee, Min Ji Song, Kwangsoo Kim, Heonyi Lee, Kyung Chul Moon, Youngsoo Kim, Minsun Jung, Ji Hye Moon, Hyebin Lee, Han Suk Ryu

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Bladder urothelial carcinoma (BUC) is the most lethal malignancy of the urinary tract. Treatment for the disease highly depends on the invasiveness of cancer cells. Therefore, a predictive biomarker needs to be identified for invasive BUC. In this study, we employed proteomics methods on urine liquid-based cytology (LBC) samples and a BUC cell line library to determine a novel predictive biomarker for invasive BUC. Furthermore, an in vitro three-dimensional (3D) invasion study for biological significance and diagnostic validation through immunocytochemistry (ICC) were also performed. The proteomic analysis suggested moesin (MSN) as a potential biomarker to predict the invasiveness of BUC. The in vitro 3D invasion study showed that inhibition of MSN significantly decreased invasiveness in BUC cell lines. Further validation using ICC ultimately confirmed moesin (MSN) as a potential biomarker to predict the invasiveness of BUC (p = 0.023). In conclusion, we suggest moesin as a potential diagnostic marker for early detection of BUC with invasion in LBC and as a potential therapeutic target.

Original language	English
Article number	1018
Journal	Cancers
Volume	12
Issue number	4
DOIs	https://doi.org/10.3390/cancers12041018
State	Published - Apr 2020
Externally published	Yes

Keywords

Biomarker
Bladder urothelial carcinoma
Invasion
Liquid-based cytology
Moesin
Proteomics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/cancers12041018

Cite this

Hwan Park, J., Lee, C., Han, D., Seok Lee, J., Min Lee, K., Ji Song, M., Kim, K., Lee, H., Chul Moon, K., Kim, Y., Jung, M., Hye Moon, J., Lee, H., & Suk Ryu, H. (2020). Moesin (MSN) as a novel proteome-based diagnostic marker for early detection of invasive bladder urothelial carcinoma in liquid-based cytology. Cancers, 12(4), Article 1018. https://doi.org/10.3390/cancers12041018

@article{c1207f2baac84db39b5e1dbc77f72202,

title = "Moesin (MSN) as a novel proteome-based diagnostic marker for early detection of invasive bladder urothelial carcinoma in liquid-based cytology",

abstract = "Bladder urothelial carcinoma (BUC) is the most lethal malignancy of the urinary tract. Treatment for the disease highly depends on the invasiveness of cancer cells. Therefore, a predictive biomarker needs to be identified for invasive BUC. In this study, we employed proteomics methods on urine liquid-based cytology (LBC) samples and a BUC cell line library to determine a novel predictive biomarker for invasive BUC. Furthermore, an in vitro three-dimensional (3D) invasion study for biological significance and diagnostic validation through immunocytochemistry (ICC) were also performed. The proteomic analysis suggested moesin (MSN) as a potential biomarker to predict the invasiveness of BUC. The in vitro 3D invasion study showed that inhibition of MSN significantly decreased invasiveness in BUC cell lines. Further validation using ICC ultimately confirmed moesin (MSN) as a potential biomarker to predict the invasiveness of BUC (p = 0.023). In conclusion, we suggest moesin as a potential diagnostic marker for early detection of BUC with invasion in LBC and as a potential therapeutic target.",

keywords = "Biomarker, Bladder urothelial carcinoma, Invasion, Liquid-based cytology, Moesin, Proteomics",

author = "\{Hwan Park\}, Jeong and Cheol Lee and Dohyun Han and \{Seok Lee\}, Jae and \{Min Lee\}, Kyung and \{Ji Song\}, Min and Kwangsoo Kim and Heonyi Lee and \{Chul Moon\}, Kyung and Youngsoo Kim and Minsun Jung and \{Hye Moon\}, Ji and Hyebin Lee and \{Suk Ryu\}, Han",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = apr,

doi = "10.3390/cancers12041018",

language = "English",

volume = "12",

journal = "Cancers",

issn = "2072-6694",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "4",

}

TY - JOUR

T1 - Moesin (MSN) as a novel proteome-based diagnostic marker for early detection of invasive bladder urothelial carcinoma in liquid-based cytology

AU - Hwan Park, Jeong

AU - Lee, Cheol

AU - Han, Dohyun

AU - Seok Lee, Jae

AU - Min Lee, Kyung

AU - Ji Song, Min

AU - Kim, Kwangsoo

AU - Lee, Heonyi

AU - Chul Moon, Kyung

AU - Kim, Youngsoo

AU - Jung, Minsun

AU - Hye Moon, Ji

AU - Lee, Hyebin

AU - Suk Ryu, Han

PY - 2020/4

Y1 - 2020/4

N2 - Bladder urothelial carcinoma (BUC) is the most lethal malignancy of the urinary tract. Treatment for the disease highly depends on the invasiveness of cancer cells. Therefore, a predictive biomarker needs to be identified for invasive BUC. In this study, we employed proteomics methods on urine liquid-based cytology (LBC) samples and a BUC cell line library to determine a novel predictive biomarker for invasive BUC. Furthermore, an in vitro three-dimensional (3D) invasion study for biological significance and diagnostic validation through immunocytochemistry (ICC) were also performed. The proteomic analysis suggested moesin (MSN) as a potential biomarker to predict the invasiveness of BUC. The in vitro 3D invasion study showed that inhibition of MSN significantly decreased invasiveness in BUC cell lines. Further validation using ICC ultimately confirmed moesin (MSN) as a potential biomarker to predict the invasiveness of BUC (p = 0.023). In conclusion, we suggest moesin as a potential diagnostic marker for early detection of BUC with invasion in LBC and as a potential therapeutic target.

AB - Bladder urothelial carcinoma (BUC) is the most lethal malignancy of the urinary tract. Treatment for the disease highly depends on the invasiveness of cancer cells. Therefore, a predictive biomarker needs to be identified for invasive BUC. In this study, we employed proteomics methods on urine liquid-based cytology (LBC) samples and a BUC cell line library to determine a novel predictive biomarker for invasive BUC. Furthermore, an in vitro three-dimensional (3D) invasion study for biological significance and diagnostic validation through immunocytochemistry (ICC) were also performed. The proteomic analysis suggested moesin (MSN) as a potential biomarker to predict the invasiveness of BUC. The in vitro 3D invasion study showed that inhibition of MSN significantly decreased invasiveness in BUC cell lines. Further validation using ICC ultimately confirmed moesin (MSN) as a potential biomarker to predict the invasiveness of BUC (p = 0.023). In conclusion, we suggest moesin as a potential diagnostic marker for early detection of BUC with invasion in LBC and as a potential therapeutic target.

KW - Biomarker

KW - Bladder urothelial carcinoma

KW - Invasion

KW - Liquid-based cytology

KW - Moesin

KW - Proteomics

UR - https://www.scopus.com/pages/publications/85083979216

U2 - 10.3390/cancers12041018

DO - 10.3390/cancers12041018

M3 - Article

AN - SCOPUS:85083979216

SN - 2072-6694

VL - 12

JO - Cancers

JF - Cancers

IS - 4

M1 - 1018

ER -

Moesin (MSN) as a novel proteome-based diagnostic marker for early detection of invasive bladder urothelial carcinoma in liquid-based cytology

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this