Abstract
We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 μg immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.
| Original language | English |
|---|---|
| Pages (from-to) | 479-483 |
| Number of pages | 5 |
| Journal | Bulletin of the Korean Chemical Society |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| State | Published - 20 Apr 2006 |
| Externally published | Yes |
Keywords
- Escherichia coli O157:H7
- Glass bead
- IgG immobilization
- Microchamber
- Pathogenic bacteria