@inproceedings{5db4c1a22bc64171b3dd693f6e0b8ed2,
title = "Microfluidic cell analysis platform: Cell culture and electrochemical lysis",
abstract = "We present an integrated microfluidic platform developed for automated cell culture and lysate analysis. Through the combination of previous technologies developed in our lab - on-chip cell culture and electrochemical lysis - we have designed, fabricated and characterized a platform that cultures cells for extended periods and lyses them on demand without the need for the addition of lysing or washing reagents. We have accomplished the successful culture and lysis of four cell lines: HeLa, MCF7, Jurkat, and CHO. Cells were cultured successfully for greater than five days, and complete on-demand lysis was demonstrated by local electrochemical generation of hydroxide. Lysate was shown to contain both nucleic acids and proteins. Additionally, the biological activity of the lysate was investigated by studying the effects of electrochemical lysis on the enzymatic activity of a model enzyme.",
keywords = "Cell-based bioassay, Electrochemical cell lysis, Microfluidic cell culture",
author = "\{Tanner Nevill\}, J. and Ryan Cooper and Megan Dueck and \{Di Carlo\}, Dino and Lee, \{Luke P.\}",
note = "Publisher Copyright: {\textcopyright} 2007 CBMS.; 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 ; Conference date: 07-10-2007 Through 11-10-2007",
year = "2007",
language = "English",
series = "Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007",
publisher = "Chemical and Biological Microsystems Society",
pages = "736--738",
editor = "Jean-Louis Viovy and Patrick Tabeling and Stephanie Descroix and Laurent Malaquin",
booktitle = "Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007",
}