TY - JOUR
T1 - Massilia chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from soil
AU - Lee, Hyosun
AU - Kim, Dong Uk
AU - Park, Sooyeon
AU - Yoon, Jung Hoon
AU - Ka, Jong Ok
N1 - Publisher Copyright:
© 2017, Springer International Publishing Switzerland.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7eT, was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33–97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA–DNA hybridisation between TA-C7eT and Massilia haematophila KACC 13771T, Massilia suwonesis KACC 12635T, and Massilia yuzhufengensis KACC 16569T revealed a low level of relatedness (<40%). The low levels of DNA–DNA hybridisation values identified strain TA-C7eT as a novel species in the genus Massilia. The major fatty acids of the strain were C16:0 (37.3%) and summed feature 3 (C16:1ω7c and/or C16:1ω6c) (40.6%). Q-8 was identified as the only isoprenoid quinone. The polar lipids profile of the strain showed the presence of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminophospholipid and three unidentified lipids. The DNA G+C content of the strain was found to be 63.2 mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain TA-C7eT represents a novel species in the genus Massilia, for which the name Massilia chloroacetimidivorans sp. nov. (=KACC 18674T = NBRC 112463T) is proposed.
AB - A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7eT, was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33–97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA–DNA hybridisation between TA-C7eT and Massilia haematophila KACC 13771T, Massilia suwonesis KACC 12635T, and Massilia yuzhufengensis KACC 16569T revealed a low level of relatedness (<40%). The low levels of DNA–DNA hybridisation values identified strain TA-C7eT as a novel species in the genus Massilia. The major fatty acids of the strain were C16:0 (37.3%) and summed feature 3 (C16:1ω7c and/or C16:1ω6c) (40.6%). Q-8 was identified as the only isoprenoid quinone. The polar lipids profile of the strain showed the presence of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminophospholipid and three unidentified lipids. The DNA G+C content of the strain was found to be 63.2 mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain TA-C7eT represents a novel species in the genus Massilia, for which the name Massilia chloroacetimidivorans sp. nov. (=KACC 18674T = NBRC 112463T) is proposed.
KW - DNA–DNA hybridisation
KW - Massilia chloroacetimidivorans
KW - Novel species
KW - Polyphasic taxonomy
UR - https://www.scopus.com/pages/publications/85027145700
U2 - 10.1007/s10482-017-0845-3
DO - 10.1007/s10482-017-0845-3
M3 - Article
C2 - 28434178
AN - SCOPUS:85027145700
SN - 0003-6072
VL - 110
SP - 751
EP - 758
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 6
ER -