Mass spectrometrical analysis of phosphoprotein enriched in astrocytes of 15 kDa in mouse hippocampi

J. H. Shin; J. M. Delabar; G. Lubec

doi:10.1007/s00726-004-0131-3

Mass spectrometrical analysis of phosphoprotein enriched in astrocytes of 15 kDa in mouse hippocampi

J. H. Shin, J. M. Delabar, G. Lubec

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2 Scopus citations

Abstract

Phosphoprotein enriched in astrocytes of 15 kDa (PEA-15) is a small protein that was first identified as an abundant phosphoprotein in brain. PEA-15 was characterised so far at the immunochemical level and by a microsequencing attempt. In order to update characterisation of this important structure by advanced methodology unambiguously identifying proteins independent of antibody availability and specificity, we used a proteomic method for this purpose: Performing protein profiling in mouse hippocampi using two dimensional gel electrophoresis with subsequent mass spectrometrical (MS/MS) identification we detected this protein and demonstrate proteomic characterisation of PEA-15 (Q62048). This study enables further specific and unambiguous determination serving as an analytical tool.

Original language	English
Pages (from-to)	339-344
Number of pages	6
Journal	Amino Acids
Volume	27
Issue number	3-4
DOIs	https://doi.org/10.1007/s00726-004-0131-3
State	Published - Dec 2004
Externally published	Yes

Keywords

Astrocytic phosphoprotein PEA-15
Hippocampus
Mouse
Two-dimensional gel electrophoresis

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10.1007/s00726-004-0131-3

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title = "Mass spectrometrical analysis of phosphoprotein enriched in astrocytes of 15 kDa in mouse hippocampi",

abstract = "Phosphoprotein enriched in astrocytes of 15 kDa (PEA-15) is a small protein that was first identified as an abundant phosphoprotein in brain. PEA-15 was characterised so far at the immunochemical level and by a microsequencing attempt. In order to update characterisation of this important structure by advanced methodology unambiguously identifying proteins independent of antibody availability and specificity, we used a proteomic method for this purpose: Performing protein profiling in mouse hippocampi using two dimensional gel electrophoresis with subsequent mass spectrometrical (MS/MS) identification we detected this protein and demonstrate proteomic characterisation of PEA-15 (Q62048). This study enables further specific and unambiguous determination serving as an analytical tool.",

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T1 - Mass spectrometrical analysis of phosphoprotein enriched in astrocytes of 15 kDa in mouse hippocampi

AU - Shin, J. H.

AU - Delabar, J. M.

AU - Lubec, G.

PY - 2004/12

Y1 - 2004/12

N2 - Phosphoprotein enriched in astrocytes of 15 kDa (PEA-15) is a small protein that was first identified as an abundant phosphoprotein in brain. PEA-15 was characterised so far at the immunochemical level and by a microsequencing attempt. In order to update characterisation of this important structure by advanced methodology unambiguously identifying proteins independent of antibody availability and specificity, we used a proteomic method for this purpose: Performing protein profiling in mouse hippocampi using two dimensional gel electrophoresis with subsequent mass spectrometrical (MS/MS) identification we detected this protein and demonstrate proteomic characterisation of PEA-15 (Q62048). This study enables further specific and unambiguous determination serving as an analytical tool.

AB - Phosphoprotein enriched in astrocytes of 15 kDa (PEA-15) is a small protein that was first identified as an abundant phosphoprotein in brain. PEA-15 was characterised so far at the immunochemical level and by a microsequencing attempt. In order to update characterisation of this important structure by advanced methodology unambiguously identifying proteins independent of antibody availability and specificity, we used a proteomic method for this purpose: Performing protein profiling in mouse hippocampi using two dimensional gel electrophoresis with subsequent mass spectrometrical (MS/MS) identification we detected this protein and demonstrate proteomic characterisation of PEA-15 (Q62048). This study enables further specific and unambiguous determination serving as an analytical tool.

KW - Astrocytic phosphoprotein PEA-15

KW - Hippocampus

KW - Mouse

KW - Two-dimensional gel electrophoresis

UR - https://www.scopus.com/pages/publications/12144270065

U2 - 10.1007/s00726-004-0131-3

DO - 10.1007/s00726-004-0131-3

M3 - Article

C2 - 15538643

AN - SCOPUS:12144270065

SN - 0939-4451

VL - 27

SP - 339

EP - 344

JO - Amino Acids

JF - Amino Acids

IS - 3-4

ER -

Mass spectrometrical analysis of phosphoprotein enriched in astrocytes of 15 kDa in mouse hippocampi

Abstract

Keywords

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