TY - JOUR
T1 - Mariniflexile ostreae sp. nov., a member of the family Flavobacteriaceae isolated from an oyster
AU - Park, Sooyeon
AU - Kang, Chul Hyung
AU - Park, Ja Min
AU - Yoon, Jung Hoon
PY - 2014/9
Y1 - 2014/9
N2 - A Gram-negative, aerobic, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated TYO-10T, was isolated from an oyster collected at Tongyoung on the South Sea, South Korea. Strain TYO-10 T was found to grow optimally at 30°C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain TYO-10T joined the cluster comprising the type strains of Mariniflexile species. Strain TYO-10T exhibited 16S rRNA gene sequence similarity values of 95.4-95.7 % to the type strains of Mariniflexile species and of less than 95.4 % to the type strains of other recognized species. Strain TYO-10T was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH as the major fatty acids. The major polar lipids of strain TYO-10 T were identified as phosphatidylethanolamine and an unidentified lipid, which is similar to those of the type strains of Mariniflexile species, but different from those of other phylogenetically related species. The DNA G+C content of strain TYO-10T was determined to be 35.9 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain TYO-10T is separated from other Mariniflexile species. On the basis of the data presented, strain TYO-10 T is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile ostreae sp. nov. is proposed. The type strain is TYO-10T (= KCTC 42113T = CECT 8622 T).
AB - A Gram-negative, aerobic, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated TYO-10T, was isolated from an oyster collected at Tongyoung on the South Sea, South Korea. Strain TYO-10 T was found to grow optimally at 30°C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain TYO-10T joined the cluster comprising the type strains of Mariniflexile species. Strain TYO-10T exhibited 16S rRNA gene sequence similarity values of 95.4-95.7 % to the type strains of Mariniflexile species and of less than 95.4 % to the type strains of other recognized species. Strain TYO-10T was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH as the major fatty acids. The major polar lipids of strain TYO-10 T were identified as phosphatidylethanolamine and an unidentified lipid, which is similar to those of the type strains of Mariniflexile species, but different from those of other phylogenetically related species. The DNA G+C content of strain TYO-10T was determined to be 35.9 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain TYO-10T is separated from other Mariniflexile species. On the basis of the data presented, strain TYO-10 T is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile ostreae sp. nov. is proposed. The type strain is TYO-10T (= KCTC 42113T = CECT 8622 T).
KW - Mariniflexile ostreae sp. nov.
KW - Novel species
KW - Oyster
KW - Polyphasic taxonomy
UR - https://www.scopus.com/pages/publications/84905578429
U2 - 10.1007/s10482-014-0227-z
DO - 10.1007/s10482-014-0227-z
M3 - Article
AN - SCOPUS:84905578429
SN - 0003-6072
VL - 106
SP - 555
EP - 563
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 3
ER -