Lysophosphatidylglycerol stimulates chemotactic migration in human natural killer cells

Seong Ho Jo; Sang Doo Kim; Jung Mo Kim; Ha Young Lee; Sun Young Lee; Jae Woong Shim; Jeanho Yun; Dong Soon Im; Yoe Sik Bae

doi:10.1016/j.bbrc.2008.05.004

Lysophosphatidylglycerol stimulates chemotactic migration in human natural killer cells

Seong Ho Jo
, Sang Doo Kim
, Jung Mo Kim
, Ha Young Lee
, Sun Young Lee
, Jae Woong Shim
, Jeanho Yun
, Dong Soon Im
, Yoe Sik Bae

Dong-A University
Pusan National University

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We observed that lysophosphatidylglycerol (LPG) stimulates chemotactic migration in human natural killer (NK) cells. The LPG-induced chemotactic migration of NK cells was completely inhibited by pertussis toxin (PTX). LPG also stimulated the extracellular signal-regulated kinase (ERK) and Akt activities in NK cells. LPG-stimulated ERK activity was inhibited by PTX, indicating the involvement of PTX-sensitive G-proteins. The preincubation of NK cells with an ERK inhibitor (PD98059) or phosphoinositide-3-kinase (PI3K) inhibitors (wortmannin and LY294002) completely inhibited LPG-induced chemotactic migration, suggesting the essential role of ERK and PI3K in the process. Moreover, LPG-induced chemotactic migration in NK cell was inhibited by Ki16425, an LPA_1/3 receptor-selective antagonist, suggesting the involvement of the Ki16425-sensitive G-protein coupled receptor (GPCR) in the process. Taken together, the results indicate that LPG stimulates chemotactic migration in NK cells through GPCR, suggesting a new function of LPG as a modulator of NK cell functioning.

Original language	English
Pages (from-to)	147-151
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.004
State	Published - 18 Jul 2008
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chemotaxis
Extracellular signal-regulated protein kinase
Lysophosphatidylglycerol
Natural killer cells

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10.1016/j.bbrc.2008.05.004

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title = "Lysophosphatidylglycerol stimulates chemotactic migration in human natural killer cells",

abstract = "We observed that lysophosphatidylglycerol (LPG) stimulates chemotactic migration in human natural killer (NK) cells. The LPG-induced chemotactic migration of NK cells was completely inhibited by pertussis toxin (PTX). LPG also stimulated the extracellular signal-regulated kinase (ERK) and Akt activities in NK cells. LPG-stimulated ERK activity was inhibited by PTX, indicating the involvement of PTX-sensitive G-proteins. The preincubation of NK cells with an ERK inhibitor (PD98059) or phosphoinositide-3-kinase (PI3K) inhibitors (wortmannin and LY294002) completely inhibited LPG-induced chemotactic migration, suggesting the essential role of ERK and PI3K in the process. Moreover, LPG-induced chemotactic migration in NK cell was inhibited by Ki16425, an LPA1/3 receptor-selective antagonist, suggesting the involvement of the Ki16425-sensitive G-protein coupled receptor (GPCR) in the process. Taken together, the results indicate that LPG stimulates chemotactic migration in NK cells through GPCR, suggesting a new function of LPG as a modulator of NK cell functioning.",

keywords = "Chemotaxis, Extracellular signal-regulated protein kinase, Lysophosphatidylglycerol, Natural killer cells",

author = "Jo, \{Seong Ho\} and Kim, \{Sang Doo\} and Kim, \{Jung Mo\} and Lee, \{Ha Young\} and Lee, \{Sun Young\} and Shim, \{Jae Woong\} and Jeanho Yun and Im, \{Dong Soon\} and Bae, \{Yoe Sik\}",

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doi = "10.1016/j.bbrc.2008.05.004",

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T1 - Lysophosphatidylglycerol stimulates chemotactic migration in human natural killer cells

AU - Jo, Seong Ho

AU - Kim, Sang Doo

AU - Kim, Jung Mo

AU - Lee, Ha Young

AU - Lee, Sun Young

AU - Shim, Jae Woong

AU - Yun, Jeanho

AU - Im, Dong Soon

AU - Bae, Yoe Sik

PY - 2008/7/18

Y1 - 2008/7/18

N2 - We observed that lysophosphatidylglycerol (LPG) stimulates chemotactic migration in human natural killer (NK) cells. The LPG-induced chemotactic migration of NK cells was completely inhibited by pertussis toxin (PTX). LPG also stimulated the extracellular signal-regulated kinase (ERK) and Akt activities in NK cells. LPG-stimulated ERK activity was inhibited by PTX, indicating the involvement of PTX-sensitive G-proteins. The preincubation of NK cells with an ERK inhibitor (PD98059) or phosphoinositide-3-kinase (PI3K) inhibitors (wortmannin and LY294002) completely inhibited LPG-induced chemotactic migration, suggesting the essential role of ERK and PI3K in the process. Moreover, LPG-induced chemotactic migration in NK cell was inhibited by Ki16425, an LPA1/3 receptor-selective antagonist, suggesting the involvement of the Ki16425-sensitive G-protein coupled receptor (GPCR) in the process. Taken together, the results indicate that LPG stimulates chemotactic migration in NK cells through GPCR, suggesting a new function of LPG as a modulator of NK cell functioning.

AB - We observed that lysophosphatidylglycerol (LPG) stimulates chemotactic migration in human natural killer (NK) cells. The LPG-induced chemotactic migration of NK cells was completely inhibited by pertussis toxin (PTX). LPG also stimulated the extracellular signal-regulated kinase (ERK) and Akt activities in NK cells. LPG-stimulated ERK activity was inhibited by PTX, indicating the involvement of PTX-sensitive G-proteins. The preincubation of NK cells with an ERK inhibitor (PD98059) or phosphoinositide-3-kinase (PI3K) inhibitors (wortmannin and LY294002) completely inhibited LPG-induced chemotactic migration, suggesting the essential role of ERK and PI3K in the process. Moreover, LPG-induced chemotactic migration in NK cell was inhibited by Ki16425, an LPA1/3 receptor-selective antagonist, suggesting the involvement of the Ki16425-sensitive G-protein coupled receptor (GPCR) in the process. Taken together, the results indicate that LPG stimulates chemotactic migration in NK cells through GPCR, suggesting a new function of LPG as a modulator of NK cell functioning.

KW - Chemotaxis

KW - Extracellular signal-regulated protein kinase

KW - Lysophosphatidylglycerol

KW - Natural killer cells

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DO - 10.1016/j.bbrc.2008.05.004

M3 - Article

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SN - 0006-291X

VL - 372

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Lysophosphatidylglycerol stimulates chemotactic migration in human natural killer cells

Abstract

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Keywords

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