Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor

Kyoung Sun Park; Ha Young Lee; Sun Young Lee; Mi Kyoung Kim; Sang Doo Kim; Jung Mo Kim; Jeanho Yun; Dong Soon Im; Yoe Sik Bae

doi:10.1016/j.febslet.2007.08.014

Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor

Kyoung Sun Park
, Ha Young Lee
, Sun Young Lee
, Mi Kyoung Kim
, Sang Doo Kim
, Jung Mo Kim
, Jeanho Yun
, Dong Soon Im
, Yoe Sik Bae

Dong-A University
Pusan National University

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

We investigated whether lysophosphatidylethanolamine (LPE) modulates cellular signaling in different cell types. SK-OV3 ovarian cancer cells and OVCAR-3 ovarian cancer cells were responsive to LPE. LPE-stimulated intracellular calcium concentration ([Ca²⁺]_i) increase was inhibited by U-73122, suggesting that LPE stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) almost completely inhibited [Ca²⁺]_i increase by LPE, indicating the involvement of PTX-sensitive G-proteins. Furthermore, we found that LPE stimulated chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. We examined the role of lysophosphatidic acid receptors on LPE-stimulated cellular responses using HepG2 cells transfected with different LPA receptors, and found that LPE failed to stimulate nuclear factor kappa B-driven luciferase. We suggest that LPE stimulates a membrane bound receptor, different from well known LPA receptors, resulting in chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells.

Original language	English
Pages (from-to)	4411-4416
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	23
DOIs	https://doi.org/10.1016/j.febslet.2007.08.014
State	Published - 18 Sep 2007
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chemotactic migration
G-protein coupled receptor
Invasion
Lysophosphatidylethanolamine
Ovarian cancer cell
Pertussis toxin-sensitive G-protein

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10.1016/j.febslet.2007.08.014

Cite this

Park, K. S., Lee, H. Y., Lee, S. Y., Kim, M. K., Kim, S. D., Kim, J. M., Yun, J., Im, D. S., & Bae, Y. S. (2007). Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor. FEBS Letters, 581(23), 4411-4416. https://doi.org/10.1016/j.febslet.2007.08.014

@article{7e99af4c4b834a089a2e377ad15a2221,

title = "Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor",

abstract = "We investigated whether lysophosphatidylethanolamine (LPE) modulates cellular signaling in different cell types. SK-OV3 ovarian cancer cells and OVCAR-3 ovarian cancer cells were responsive to LPE. LPE-stimulated intracellular calcium concentration ([Ca2+]i) increase was inhibited by U-73122, suggesting that LPE stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) almost completely inhibited [Ca2+]i increase by LPE, indicating the involvement of PTX-sensitive G-proteins. Furthermore, we found that LPE stimulated chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. We examined the role of lysophosphatidic acid receptors on LPE-stimulated cellular responses using HepG2 cells transfected with different LPA receptors, and found that LPE failed to stimulate nuclear factor kappa B-driven luciferase. We suggest that LPE stimulates a membrane bound receptor, different from well known LPA receptors, resulting in chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells.",

keywords = "Chemotactic migration, G-protein coupled receptor, Invasion, Lysophosphatidylethanolamine, Ovarian cancer cell, Pertussis toxin-sensitive G-protein",

author = "Park, \{Kyoung Sun\} and Lee, \{Ha Young\} and Lee, \{Sun Young\} and Kim, \{Mi Kyoung\} and Kim, \{Sang Doo\} and Kim, \{Jung Mo\} and Jeanho Yun and Im, \{Dong Soon\} and Bae, \{Yoe Sik\}",

year = "2007",

month = sep,

day = "18",

doi = "10.1016/j.febslet.2007.08.014",

language = "English",

volume = "581",

pages = "4411--4416",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "23",

}

Park, KS, Lee, HY, Lee, SY, Kim, MK, Kim, SD, Kim, JM, Yun, J, Im, DS & Bae, YS 2007, 'Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor', FEBS Letters, vol. 581, no. 23, pp. 4411-4416. https://doi.org/10.1016/j.febslet.2007.08.014

Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor. / Park, Kyoung Sun; Lee, Ha Young; Lee, Sun Young et al.
In: FEBS Letters, Vol. 581, No. 23, 18.09.2007, p. 4411-4416.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells

T2 - Involvement of pertussis toxin-sensitive G-protein coupled receptor

AU - Park, Kyoung Sun

AU - Lee, Ha Young

AU - Lee, Sun Young

AU - Kim, Mi Kyoung

AU - Kim, Sang Doo

AU - Kim, Jung Mo

AU - Yun, Jeanho

AU - Im, Dong Soon

AU - Bae, Yoe Sik

PY - 2007/9/18

Y1 - 2007/9/18

N2 - We investigated whether lysophosphatidylethanolamine (LPE) modulates cellular signaling in different cell types. SK-OV3 ovarian cancer cells and OVCAR-3 ovarian cancer cells were responsive to LPE. LPE-stimulated intracellular calcium concentration ([Ca2+]i) increase was inhibited by U-73122, suggesting that LPE stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) almost completely inhibited [Ca2+]i increase by LPE, indicating the involvement of PTX-sensitive G-proteins. Furthermore, we found that LPE stimulated chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. We examined the role of lysophosphatidic acid receptors on LPE-stimulated cellular responses using HepG2 cells transfected with different LPA receptors, and found that LPE failed to stimulate nuclear factor kappa B-driven luciferase. We suggest that LPE stimulates a membrane bound receptor, different from well known LPA receptors, resulting in chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells.

AB - We investigated whether lysophosphatidylethanolamine (LPE) modulates cellular signaling in different cell types. SK-OV3 ovarian cancer cells and OVCAR-3 ovarian cancer cells were responsive to LPE. LPE-stimulated intracellular calcium concentration ([Ca2+]i) increase was inhibited by U-73122, suggesting that LPE stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) almost completely inhibited [Ca2+]i increase by LPE, indicating the involvement of PTX-sensitive G-proteins. Furthermore, we found that LPE stimulated chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells. We examined the role of lysophosphatidic acid receptors on LPE-stimulated cellular responses using HepG2 cells transfected with different LPA receptors, and found that LPE failed to stimulate nuclear factor kappa B-driven luciferase. We suggest that LPE stimulates a membrane bound receptor, different from well known LPA receptors, resulting in chemotactic migration and cellular invasion in SK-OV3 ovarian cancer cells.

KW - Chemotactic migration

KW - G-protein coupled receptor

KW - Invasion

KW - Lysophosphatidylethanolamine

KW - Ovarian cancer cell

KW - Pertussis toxin-sensitive G-protein

UR - https://www.scopus.com/pages/publications/34548442406

U2 - 10.1016/j.febslet.2007.08.014

DO - 10.1016/j.febslet.2007.08.014

M3 - Article

C2 - 17719584

AN - SCOPUS:34548442406

SN - 0014-5793

VL - 581

SP - 4411

EP - 4416

JO - FEBS Letters

JF - FEBS Letters

IS - 23

ER -

Lysophosphatidylethanolamine stimulates chemotactic migration and cellular invasion in SK-OV3 human ovarian cancer cells: Involvement of pertussis toxin-sensitive G-protein coupled receptor

Abstract

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Keywords

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