Lysophosphatidic acid is a mediator of Trp-Lys-Tyr-Met-Val-D-Met-induced calcium influx

Ha Young Lee; Hyun Kyu Kang; Hye Ran Yoon; Jong Young Kwak; Yoe Sik Bae

doi:10.1016/j.bbrc.2004.09.072

Lysophosphatidic acid is a mediator of Trp-Lys-Tyr-Met-Val-D-Met-induced calcium influx

Ha Young Lee
, Hyun Kyu Kang
, Hye Ran Yoon
, Jong Young Kwak
, Yoe Sik Bae

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Intracellular calcium (Ca ²⁺) homeostasis is very strictly regulated, and the activation of G-protein-coupled receptor (GPCR) can cause two different calcium changes, intracellular calcium release, and calcium influx. In this study, we investigated the possible role of lysophosphatidic acid (LPA) on GPCR-induced Ca ²⁺ signaling. The addition of exogenous LPA induced dramatic Ca ²⁺ influx but not intracellular Ca ²⁺ release in U937 cells. LPA-induced Ca ²⁺ influx was not affected by pertussis toxin and phospholipase C inhibitor (U73122), ruling out the involvement of pertussis toxin-sensitive G-proteins, and phospholipase C. Stimulation of U937 cells with Trp-Lys-Tyr-Met-Val-d-Met (WKYMVm), which binds to formyl peptide receptor like 1, enhanced phospholipase A ₂ and phospholipase D activation, indicating LPA formation. The inhibition of LPA synthesis by phospholipase A ₂-specific inhibitor (MAFP) or n-butanol significantly inhibited WKYMVm-induced Ca ²⁺ influx, suggesting a crucial role for LPA in the process. Taken together, we suggest that LPA mediates WKYMVm-induced Ca ²⁺ influx.

Original language	English
Pages (from-to)	458-465
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	324
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2004.09.072
State	Published - 5 Nov 2004
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Ca influx
Lysophosphatidic acid
Phospholipase A
Phospholipase D

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10.1016/j.bbrc.2004.09.072

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@article{68be36a4d9ad48ee838ca074ee60a35e,

title = "Lysophosphatidic acid is a mediator of Trp-Lys-Tyr-Met-Val-D-Met-induced calcium influx",

abstract = "Intracellular calcium (Ca 2+) homeostasis is very strictly regulated, and the activation of G-protein-coupled receptor (GPCR) can cause two different calcium changes, intracellular calcium release, and calcium influx. In this study, we investigated the possible role of lysophosphatidic acid (LPA) on GPCR-induced Ca 2+ signaling. The addition of exogenous LPA induced dramatic Ca 2+ influx but not intracellular Ca 2+ release in U937 cells. LPA-induced Ca 2+ influx was not affected by pertussis toxin and phospholipase C inhibitor (U73122), ruling out the involvement of pertussis toxin-sensitive G-proteins, and phospholipase C. Stimulation of U937 cells with Trp-Lys-Tyr-Met-Val-d-Met (WKYMVm), which binds to formyl peptide receptor like 1, enhanced phospholipase A 2 and phospholipase D activation, indicating LPA formation. The inhibition of LPA synthesis by phospholipase A 2-specific inhibitor (MAFP) or n-butanol significantly inhibited WKYMVm-induced Ca 2+ influx, suggesting a crucial role for LPA in the process. Taken together, we suggest that LPA mediates WKYMVm-induced Ca 2+ influx.",

keywords = "Ca influx, Lysophosphatidic acid, Phospholipase A, Phospholipase D",

author = "Lee, \{Ha Young\} and Kang, \{Hyun Kyu\} and Yoon, \{Hye Ran\} and Kwak, \{Jong Young\} and Bae, \{Yoe Sik\}",

year = "2004",

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doi = "10.1016/j.bbrc.2004.09.072",

language = "English",

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TY - JOUR

T1 - Lysophosphatidic acid is a mediator of Trp-Lys-Tyr-Met-Val-D-Met-induced calcium influx

AU - Lee, Ha Young

AU - Kang, Hyun Kyu

AU - Yoon, Hye Ran

AU - Kwak, Jong Young

AU - Bae, Yoe Sik

PY - 2004/11/5

Y1 - 2004/11/5

N2 - Intracellular calcium (Ca 2+) homeostasis is very strictly regulated, and the activation of G-protein-coupled receptor (GPCR) can cause two different calcium changes, intracellular calcium release, and calcium influx. In this study, we investigated the possible role of lysophosphatidic acid (LPA) on GPCR-induced Ca 2+ signaling. The addition of exogenous LPA induced dramatic Ca 2+ influx but not intracellular Ca 2+ release in U937 cells. LPA-induced Ca 2+ influx was not affected by pertussis toxin and phospholipase C inhibitor (U73122), ruling out the involvement of pertussis toxin-sensitive G-proteins, and phospholipase C. Stimulation of U937 cells with Trp-Lys-Tyr-Met-Val-d-Met (WKYMVm), which binds to formyl peptide receptor like 1, enhanced phospholipase A 2 and phospholipase D activation, indicating LPA formation. The inhibition of LPA synthesis by phospholipase A 2-specific inhibitor (MAFP) or n-butanol significantly inhibited WKYMVm-induced Ca 2+ influx, suggesting a crucial role for LPA in the process. Taken together, we suggest that LPA mediates WKYMVm-induced Ca 2+ influx.

AB - Intracellular calcium (Ca 2+) homeostasis is very strictly regulated, and the activation of G-protein-coupled receptor (GPCR) can cause two different calcium changes, intracellular calcium release, and calcium influx. In this study, we investigated the possible role of lysophosphatidic acid (LPA) on GPCR-induced Ca 2+ signaling. The addition of exogenous LPA induced dramatic Ca 2+ influx but not intracellular Ca 2+ release in U937 cells. LPA-induced Ca 2+ influx was not affected by pertussis toxin and phospholipase C inhibitor (U73122), ruling out the involvement of pertussis toxin-sensitive G-proteins, and phospholipase C. Stimulation of U937 cells with Trp-Lys-Tyr-Met-Val-d-Met (WKYMVm), which binds to formyl peptide receptor like 1, enhanced phospholipase A 2 and phospholipase D activation, indicating LPA formation. The inhibition of LPA synthesis by phospholipase A 2-specific inhibitor (MAFP) or n-butanol significantly inhibited WKYMVm-induced Ca 2+ influx, suggesting a crucial role for LPA in the process. Taken together, we suggest that LPA mediates WKYMVm-induced Ca 2+ influx.

KW - Ca influx

KW - Lysophosphatidic acid

KW - Phospholipase A

KW - Phospholipase D

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U2 - 10.1016/j.bbrc.2004.09.072

DO - 10.1016/j.bbrc.2004.09.072

M3 - Article

C2 - 15465041

AN - SCOPUS:4744364045

SN - 0006-291X

VL - 324

SP - 458

EP - 465

JO - Biochemical and Biophysical Research Communications

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IS - 1

ER -

Lysophosphatidic acid is a mediator of Trp-Lys-Tyr-Met-Val-D-Met-induced calcium influx

Abstract

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Keywords

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