Litorivivens lipolytica gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat

A Gram-stain-negative, flagellated, aerobic and rod-shaped or ovoid bacterial strain, designated HJTF-7^T, was isolated from a tidal flat on the South Sea of South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain HJTF-7^T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees, based on 16S rRNA gene sequences, showed that strain HJTF-7^T joined the cluster comprising the type strains of species of the genera Spongiibacter and Zhongshania. Strain HJTF-7^Texhibited 16S rRNA gene sequence similarities of 90.4–92.5 % to the type strains of species of the genera Spongiibacter and Zhongshania and of less than 91.5 % to the type strains of other recognized species. Strain HJTF-7^Tcontained Q-8 as the predominant ubiquinone. The major fatty acids were iso-C_17 : 1ω9c, iso-C_15 : 0, iso-C_17 : 0, iso-C_11 : 0 3-OH and C_17 : 1ω8c and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The fatty acid and polar lipid profiles of strain HJTF-7^T were distinct from those of members of the genera Spongiibacter and Zhongshania. The DNA G+C content of strain HJTF-7^T was 55.9 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain HJTF-7^Trepresents a novel genus and species within the class Gammaproteobacteria, for which the name Litorivivens lipolytica gen. nov., sp. nov. is proposed. The type strain of Litorivivens lipolytica is HJTF-7^T ( = KCTC 42157^T = CECT 8654^T).