Lacinutrix undariae sp. Nov., isolated from a brown algae reservoir

A Gram-stain-negative, aerobic, non-flagellated, non-gliding and ovoid or rod-shaped bacterium, designated strain W-BA8^T, was isolated from a brown algae reservoir on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain W-BA8^T grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 1.0-2.0% (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain W-BA8^T clustered with the type strains of species of the genus Lacinutrix. Strain W-BA8^T exhibited 16S rRNA gene sequence similarity values of 94.9-96.5% to the type strains of Lacinutrix species and of less than 95.8% to the type strains of other recognized species. Strain W-BA8^T contained MK-6 as the predominant menaquinone and iso-C_15:O, iso-C_15:1 G, iso-C_15:O 3-OH and iso-C_17:O 3-OH as major fatty acids. The polar lipid profile of strain W-BA8^T contained phosphatidylethanolamine, two unidentified lipids and one unidentified glycolipid as major components. The DNA G + C content of strain W-BA8^T was 35 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain W-BA8^T is separated from other species of the genus Lacinutrix. On the basis of the data presented, strain W-BA8^T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix undariae sp. nov. is proposed. The type strain is W-BA8^T (=KCTC 42176^T=CECT 8671^T).