Janibacter melonis sp. nov., isolated from abnormally spoiled oriental melon in Korea

Two Gram-positive bacterial strains, CM2104^T and CM2110, isolated from the inner part of abnormally spoiled oriental melon (Cucumis melo) in Korea, were subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan of strains CM2104^T and CM2110 contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H₄). The major fatty acids detected in the two strains were iso-C_16:0, C_17:1ω8c and C_18:1ω9c or C_17:0. The DNA G + C content of the two strains was 73 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed a coherent cluster with a clade comprising two Janibacter species, Janibacter limosus and Janibacter terrae. Strains CM2104^T and CM2110 exhibited a 16S rRNA gene sequence similarity value of 99.7% and a mean DNA-DNA relatedness level of 89%. Strains CM2104^T and CM2110 showed 16S rRNA gene sequence similarity levels of 97.8-98.4% to the type strains of J. limosus and J. terrae. DNA-DNA relatedness between strains CM2104^T and CM2110 and the type strains of these two Janibacter species was 7-11%. On the basis of the phenotypic and phylogenetic data and genomic distinctiveness, strains CM2104^T and CM2110 should be placed within the genus Janibacter as members of a novel species, for which the name Janibacter melonis sp. nov. is proposed. The type strain is CM2104^T (= KCTC 9987^T =DSM 16063^T = JCM 12321^T).