Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein

Woo Sung Kim; Hee Do Chae; Inji Jung; Won Kyu Lee; Woo Jun Lee; Jisun Lee; Yejin Gong; Dohyun Lee; Byeong Won Kim; Jin Koo Kim; Jaehyeon Hwang; Dae Hyuk Kweon; Sang Taek Jung; Jung Hyun Na

doi:10.1016/j.fsi.2023.108807

Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein

Woo Sung Kim, Hee Do Chae, Inji Jung, Won Kyu Lee, Woo Jun Lee, Jisun Lee, Yejin Gong, Dohyun Lee, Byeong Won Kim, Jin Koo Kim, Jaehyeon Hwang, Dae Hyuk Kweon, Sang Taek Jung, Jung Hyun Na

Department of Integrative Biotechnology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The COVID-19 pandemic has significantly impacted human health for three years. To mitigate the spread of SARS-CoV-2, the development of neutralizing antibodies has been accelerated, including the exploration of alternative antibody formats such as single-domain antibodies. In this study, we identified variable new antigen receptors (VNARs) specific for the receptor binding domain (RBD) of SARS-CoV-2 by immunizing a banded houndshark (Triakis scyllium) with recombinant wild-type RBD. Notably, the CoV₂NAR-1 clone showed high binding affinities in the nanomolar range to various RBDs and demonstrated neutralizing activity against SARS-CoV-2 pseudoviruses. These results highlight the potential of the banded houndshark as an animal model for the development of VNAR-based therapeutics or diagnostics against future pandemics.

Original language	English
Article number	108807
Journal	Fish and Shellfish Immunology
Volume	138
DOIs	https://doi.org/10.1016/j.fsi.2023.108807
State	Published - Jul 2023

Keywords

Banded houndshark (Triakis scyllium)
COVID-19
Phage display immune library
SARS-CoV-2
Single domain antibody
Variable new antigen receptor (VNAR)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Kim, W. S., Chae, H. D., Jung, I., Lee, W. K., Lee, W. J., Lee, J., Gong, Y., Lee, D., Kim, B. W., Kim, J. K., Hwang, J., Kweon, D. H., Jung, S. T., & Na, J. H. (2023). Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein. Fish and Shellfish Immunology, 138, Article 108807. https://doi.org/10.1016/j.fsi.2023.108807

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title = "Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein",

abstract = "The COVID-19 pandemic has significantly impacted human health for three years. To mitigate the spread of SARS-CoV-2, the development of neutralizing antibodies has been accelerated, including the exploration of alternative antibody formats such as single-domain antibodies. In this study, we identified variable new antigen receptors (VNARs) specific for the receptor binding domain (RBD) of SARS-CoV-2 by immunizing a banded houndshark (Triakis scyllium) with recombinant wild-type RBD. Notably, the CoV2NAR-1 clone showed high binding affinities in the nanomolar range to various RBDs and demonstrated neutralizing activity against SARS-CoV-2 pseudoviruses. These results highlight the potential of the banded houndshark as an animal model for the development of VNAR-based therapeutics or diagnostics against future pandemics.",

keywords = "Banded houndshark (Triakis scyllium), COVID-19, Phage display immune library, SARS-CoV-2, Single domain antibody, Variable new antigen receptor (VNAR)",

author = "Kim, \{Woo Sung\} and Chae, \{Hee Do\} and Inji Jung and Lee, \{Won Kyu\} and Lee, \{Woo Jun\} and Jisun Lee and Yejin Gong and Dohyun Lee and Kim, \{Byeong Won\} and Kim, \{Jin Koo\} and Jaehyeon Hwang and Kweon, \{Dae Hyuk\} and Jung, \{Sang Taek\} and Na, \{Jung Hyun\}",

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year = "2023",

month = jul,

doi = "10.1016/j.fsi.2023.108807",

language = "English",

volume = "138",

journal = "Fish and Shellfish Immunology",

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AU - Kim, Woo Sung

AU - Chae, Hee Do

AU - Jung, Inji

AU - Lee, Won Kyu

AU - Lee, Woo Jun

AU - Lee, Jisun

AU - Gong, Yejin

AU - Lee, Dohyun

AU - Kim, Byeong Won

AU - Kim, Jin Koo

AU - Hwang, Jaehyeon

AU - Kweon, Dae Hyuk

AU - Jung, Sang Taek

AU - Na, Jung Hyun

PY - 2023/7

Y1 - 2023/7

N2 - The COVID-19 pandemic has significantly impacted human health for three years. To mitigate the spread of SARS-CoV-2, the development of neutralizing antibodies has been accelerated, including the exploration of alternative antibody formats such as single-domain antibodies. In this study, we identified variable new antigen receptors (VNARs) specific for the receptor binding domain (RBD) of SARS-CoV-2 by immunizing a banded houndshark (Triakis scyllium) with recombinant wild-type RBD. Notably, the CoV2NAR-1 clone showed high binding affinities in the nanomolar range to various RBDs and demonstrated neutralizing activity against SARS-CoV-2 pseudoviruses. These results highlight the potential of the banded houndshark as an animal model for the development of VNAR-based therapeutics or diagnostics against future pandemics.

AB - The COVID-19 pandemic has significantly impacted human health for three years. To mitigate the spread of SARS-CoV-2, the development of neutralizing antibodies has been accelerated, including the exploration of alternative antibody formats such as single-domain antibodies. In this study, we identified variable new antigen receptors (VNARs) specific for the receptor binding domain (RBD) of SARS-CoV-2 by immunizing a banded houndshark (Triakis scyllium) with recombinant wild-type RBD. Notably, the CoV2NAR-1 clone showed high binding affinities in the nanomolar range to various RBDs and demonstrated neutralizing activity against SARS-CoV-2 pseudoviruses. These results highlight the potential of the banded houndshark as an animal model for the development of VNAR-based therapeutics or diagnostics against future pandemics.

KW - Banded houndshark (Triakis scyllium)

KW - COVID-19

KW - Phage display immune library

KW - SARS-CoV-2

KW - Single domain antibody

KW - Variable new antigen receptor (VNAR)

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SN - 1050-4648

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ER -

Isolation and characterization of single domain antibodies from banded houndshark (Triakis scyllium) targeting SARS-CoV-2 spike RBD protein

Abstract

Keywords

UN SDGs

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