TY - JOUR
T1 - Inhibitory effects of Lactobacillus acidophilus lysates on the cytotoxic activity of shiga-like toxin 2 produced from Escherichia coli O157:H7
AU - Kim, Y.
AU - Han, K. S.
AU - Imm, J. Y.
AU - Oh, S.
AU - You, S.
AU - Park, S.
AU - Kim, S. H.
PY - 2006/11
Y1 - 2006/11
N2 - Aims: The purpose of this study was to characterize the degree to which four cell lysates obtained from Lactobacillus acidophilus strains affected the cytotoxic activity of Escherichia coli O157:H7 in vitro and in vivo. Methods and Results: In a cytotoxic inhibition test that used 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide and toxin-binding ELISA assays, the activity of shiga-like toxin 2 (Stx-2) was inhibited profoundly by the cell lysates (10 mg ml-1) from two strains of L. acidophilus A4 and 30SC (>85% of survival rates compared with the control) among the five strains tested. In particular, a significant decline in the virulence level of E. coli O157:H7, under the presence of the cell lysates of L. acidophilus A4, was observed by killing assay of Caenorhabditis elegans in vivo model. Conclusions: According to our results, L. acidophilus strains might be capable of attenuating the virulence of Stx-2 produced from E. coli O157:H7. Significance and Impact of the Study: The cell lysates of L. acidophilus can be applied to a variety of foods, and can be used as adjuncts for the inhibition of Stx-2-mediated cytotoxicity.
AB - Aims: The purpose of this study was to characterize the degree to which four cell lysates obtained from Lactobacillus acidophilus strains affected the cytotoxic activity of Escherichia coli O157:H7 in vitro and in vivo. Methods and Results: In a cytotoxic inhibition test that used 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide and toxin-binding ELISA assays, the activity of shiga-like toxin 2 (Stx-2) was inhibited profoundly by the cell lysates (10 mg ml-1) from two strains of L. acidophilus A4 and 30SC (>85% of survival rates compared with the control) among the five strains tested. In particular, a significant decline in the virulence level of E. coli O157:H7, under the presence of the cell lysates of L. acidophilus A4, was observed by killing assay of Caenorhabditis elegans in vivo model. Conclusions: According to our results, L. acidophilus strains might be capable of attenuating the virulence of Stx-2 produced from E. coli O157:H7. Significance and Impact of the Study: The cell lysates of L. acidophilus can be applied to a variety of foods, and can be used as adjuncts for the inhibition of Stx-2-mediated cytotoxicity.
KW - Caenorhabditis elegans
KW - Cytotoxicity
KW - Escherichia coli O157:H7
KW - Lactobacillus acidophilus
KW - Shiga-like toxin 2
UR - https://www.scopus.com/pages/publications/33750003354
U2 - 10.1111/j.1472-765X.2006.02005.x
DO - 10.1111/j.1472-765X.2006.02005.x
M3 - Article
C2 - 17032223
AN - SCOPUS:33750003354
SN - 0266-8254
VL - 43
SP - 502
EP - 507
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 5
ER -
