Inhibitory effect of Uncaria sinensis on human aortic smooth muscle cell migration is based on matrix metalloproteinase-9 inhibitory activity

Ki Tae Ha; Kyung Woon Kim; Seok Jong Suh; Chang Geun Kwak; June Ki Kim; Cheorl Ho Kim

doi:10.1016/j.etap.2007.05.008

Inhibitory effect of Uncaria sinensis on human aortic smooth muscle cell migration is based on matrix metalloproteinase-9 inhibitory activity

Ki Tae Ha
, Kyung Woon Kim
, Seok Jong Suh
, Chang Geun Kwak
, June Ki Kim
, Cheorl Ho Kim

Department of Biological Sciences

Dongguk University
Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Medicinal extracts of Cho-Deung-san and Uncaria sinensis Havil. (UR) have previously been shown to have inhibitory effects on migration of vascular smooth muscle cells (VSMC) and matrix metalloproteinase (MMP)-2/9 production, which play key roles in the development of atherosclerosis. In this study, we have more extensively investigated the inhibitory effect of UR on MMP-9 activity and TNF-α induced human aortic smooth muscle cells (HASMC) migration. The result from gelatin zymography showed that UR inhibited MMP-9 activity in a dose-dependent manner (IC₅₀ = 55 μg/ml). In addition, UR strongly inhibited the migration of HASMC induced by TNF-α treatment (IC₅₀ = 125 μg/ml), although it has very low cytotoxic effect on HASMC (IC₅₀ > 500 μg/ml). These results suggest that UR is a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and VSMC migration.

Original language	English
Pages (from-to)	218-222
Number of pages	5
Journal	Environmental Toxicology and Pharmacology
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.etap.2007.05.008
State	Published - Nov 2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Human aortic smooth muscle cells
Matrix metalloproteinase-9
Migration
Uncaria sinensis
Zymography

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10.1016/j.etap.2007.05.008

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@article{12d3a3d240a6457ea60ecb93a3eab9c5,

title = "Inhibitory effect of Uncaria sinensis on human aortic smooth muscle cell migration is based on matrix metalloproteinase-9 inhibitory activity",

abstract = "Medicinal extracts of Cho-Deung-san and Uncaria sinensis Havil. (UR) have previously been shown to have inhibitory effects on migration of vascular smooth muscle cells (VSMC) and matrix metalloproteinase (MMP)-2/9 production, which play key roles in the development of atherosclerosis. In this study, we have more extensively investigated the inhibitory effect of UR on MMP-9 activity and TNF-α induced human aortic smooth muscle cells (HASMC) migration. The result from gelatin zymography showed that UR inhibited MMP-9 activity in a dose-dependent manner (IC50 = 55 μg/ml). In addition, UR strongly inhibited the migration of HASMC induced by TNF-α treatment (IC50 = 125 μg/ml), although it has very low cytotoxic effect on HASMC (IC50 > 500 μg/ml). These results suggest that UR is a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and VSMC migration.",

keywords = "Human aortic smooth muscle cells, Matrix metalloproteinase-9, Migration, Uncaria sinensis, Zymography",

author = "Ha, \{Ki Tae\} and Kim, \{Kyung Woon\} and Suh, \{Seok Jong\} and Kwak, \{Chang Geun\} and Kim, \{June Ki\} and Kim, \{Cheorl Ho\}",

year = "2007",

month = nov,

doi = "10.1016/j.etap.2007.05.008",

language = "English",

volume = "24",

pages = "218--222",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Inhibitory effect of Uncaria sinensis on human aortic smooth muscle cell migration is based on matrix metalloproteinase-9 inhibitory activity

AU - Ha, Ki Tae

AU - Kim, Kyung Woon

AU - Suh, Seok Jong

AU - Kwak, Chang Geun

AU - Kim, June Ki

AU - Kim, Cheorl Ho

PY - 2007/11

Y1 - 2007/11

N2 - Medicinal extracts of Cho-Deung-san and Uncaria sinensis Havil. (UR) have previously been shown to have inhibitory effects on migration of vascular smooth muscle cells (VSMC) and matrix metalloproteinase (MMP)-2/9 production, which play key roles in the development of atherosclerosis. In this study, we have more extensively investigated the inhibitory effect of UR on MMP-9 activity and TNF-α induced human aortic smooth muscle cells (HASMC) migration. The result from gelatin zymography showed that UR inhibited MMP-9 activity in a dose-dependent manner (IC50 = 55 μg/ml). In addition, UR strongly inhibited the migration of HASMC induced by TNF-α treatment (IC50 = 125 μg/ml), although it has very low cytotoxic effect on HASMC (IC50 > 500 μg/ml). These results suggest that UR is a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and VSMC migration.

AB - Medicinal extracts of Cho-Deung-san and Uncaria sinensis Havil. (UR) have previously been shown to have inhibitory effects on migration of vascular smooth muscle cells (VSMC) and matrix metalloproteinase (MMP)-2/9 production, which play key roles in the development of atherosclerosis. In this study, we have more extensively investigated the inhibitory effect of UR on MMP-9 activity and TNF-α induced human aortic smooth muscle cells (HASMC) migration. The result from gelatin zymography showed that UR inhibited MMP-9 activity in a dose-dependent manner (IC50 = 55 μg/ml). In addition, UR strongly inhibited the migration of HASMC induced by TNF-α treatment (IC50 = 125 μg/ml), although it has very low cytotoxic effect on HASMC (IC50 > 500 μg/ml). These results suggest that UR is a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and VSMC migration.

KW - Human aortic smooth muscle cells

KW - Matrix metalloproteinase-9

KW - Migration

KW - Uncaria sinensis

KW - Zymography

UR - https://www.scopus.com/pages/publications/34848918865

U2 - 10.1016/j.etap.2007.05.008

DO - 10.1016/j.etap.2007.05.008

M3 - Article

AN - SCOPUS:34848918865

SN - 1382-6689

VL - 24

SP - 218

EP - 222

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

IS - 3

ER -

Inhibitory effect of Uncaria sinensis on human aortic smooth muscle cell migration is based on matrix metalloproteinase-9 inhibitory activity

Abstract

UN SDGs

Keywords

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