Inhibitory effect of sulforaphane on secretory group IIA phospholipase A₂

Background and Objective: The expression of secretory group IIA phospholipase A2 (sPLA2-IIA) has been shown to be elevated in various inflammatory diseases and Lipopolysaccharide (LPS) up-regulates the expression of sPLA2-IIA in Human umbilical vein endothelial cells (HUVECs). Sulforaphane (SFN), a natural isothiocyanate present in cruciferous vegetables such as broccoli and cabbage, is effective in preventing carcinogenesis, diabetes and inflammatory responses. Here, SFN was examined for its effects on the expression and activity of sPLA₂-IIA in HUVECs and in mouse models of sepsis. Materials and Methods: After HUVECs were activated with LPS, cells were post-treated with SFN. In vivo, LPS-injected or Cecal ligation and puncture (CLP) operated mice were administrated SFN. Then, the effects of SFN on the activity and expression of sPLA2-IIA were determined by Enzyme-linked immunosorbent assay (ELISA). The effects of SFN on the activities of cytosolic phospholipase A2 (cPLA2) and Extracellular signal-regulated kinase (ERK)1/2 were monitored. Statistical relevance was determined by one-way analysis of variance (ANOVA). p<0.05 were considered to indicate significance Results: Post-treatment of cells or mice with SFN inhibited LPS- or CLP-induced expression and activity of sPLA₂-IIA. SFN also suppressed the activation of cPLA2 and ERK1/2 by LPS. Conclusion: It is concluded that, SFN inhibited LPS-mediated expression of sPLA₂-IIA by suppression of cPLA2 and ERK1/2.