Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells

Dong Il Park; Jae Hun Lee; Sung Kwon Moon; Cheorl Ho Kim; Yong Tae Lee; Jae Hun Cheong; Byung Tae Choi; Yung Hyun Choi

doi:10.1016/j.phrs.2004.11.003

Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells

Dong Il Park, Jae Hun Lee, Sung Kwon Moon, Cheorl Ho Kim, Yong Tae Lee, Jae Hun Cheong, Byung Tae Choi, Yung Hyun Choi

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The objective of the present study was to investigate the effect of aqueous extract from the root of Platycodon grandiflorum (AEPG) on the cell growth and apoptosis in human lung carcinoma cell line A549. Exposure of A549 cells to AEPG resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. This increase in apoptosis was associated with a decrease in Bcl-2 expression, an increase of Bax and an activation of caspase-3. AEPG treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by AEPG treatment. These findings suggest that the apoptotic events by AEPG were associated with the diminished telomerase activity and down-regulation of Bcl-2 expression.

Original language	English
Pages (from-to)	437-443
Number of pages	7
Journal	Pharmacological Research
Volume	51
Issue number	5
DOIs	https://doi.org/10.1016/j.phrs.2004.11.003
State	Published - May 2005
Externally published	Yes

Keywords

Apoptosis
Bcl-2
hTERT
Platycodon grandiflorum
Telomerase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.phrs.2004.11.003

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@article{315d8812c0f041ed8244f2b72bce53a0,

title = "Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells",

abstract = "The objective of the present study was to investigate the effect of aqueous extract from the root of Platycodon grandiflorum (AEPG) on the cell growth and apoptosis in human lung carcinoma cell line A549. Exposure of A549 cells to AEPG resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. This increase in apoptosis was associated with a decrease in Bcl-2 expression, an increase of Bax and an activation of caspase-3. AEPG treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by AEPG treatment. These findings suggest that the apoptotic events by AEPG were associated with the diminished telomerase activity and down-regulation of Bcl-2 expression.",

keywords = "Apoptosis, Bcl-2, hTERT, Platycodon grandiflorum, Telomerase",

author = "Park, \{Dong Il\} and Lee, \{Jae Hun\} and Moon, \{Sung Kwon\} and Kim, \{Cheorl Ho\} and Lee, \{Yong Tae\} and Cheong, \{Jae Hun\} and Choi, \{Byung Tae\} and Choi, \{Yung Hyun\}",

year = "2005",

month = may,

doi = "10.1016/j.phrs.2004.11.003",

language = "English",

volume = "51",

pages = "437--443",

journal = "Pharmacological Research",

issn = "1043-6618",

publisher = "Academic Press",

number = "5",

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TY - JOUR

T1 - Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells

AU - Park, Dong Il

AU - Lee, Jae Hun

AU - Moon, Sung Kwon

AU - Kim, Cheorl Ho

AU - Lee, Yong Tae

AU - Cheong, Jae Hun

AU - Choi, Byung Tae

AU - Choi, Yung Hyun

PY - 2005/5

Y1 - 2005/5

N2 - The objective of the present study was to investigate the effect of aqueous extract from the root of Platycodon grandiflorum (AEPG) on the cell growth and apoptosis in human lung carcinoma cell line A549. Exposure of A549 cells to AEPG resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. This increase in apoptosis was associated with a decrease in Bcl-2 expression, an increase of Bax and an activation of caspase-3. AEPG treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by AEPG treatment. These findings suggest that the apoptotic events by AEPG were associated with the diminished telomerase activity and down-regulation of Bcl-2 expression.

AB - The objective of the present study was to investigate the effect of aqueous extract from the root of Platycodon grandiflorum (AEPG) on the cell growth and apoptosis in human lung carcinoma cell line A549. Exposure of A549 cells to AEPG resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. This increase in apoptosis was associated with a decrease in Bcl-2 expression, an increase of Bax and an activation of caspase-3. AEPG treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by AEPG treatment. These findings suggest that the apoptotic events by AEPG were associated with the diminished telomerase activity and down-regulation of Bcl-2 expression.

KW - Apoptosis

KW - Bcl-2

KW - hTERT

KW - Platycodon grandiflorum

KW - Telomerase

UR - https://www.scopus.com/pages/publications/14644399849

U2 - 10.1016/j.phrs.2004.11.003

DO - 10.1016/j.phrs.2004.11.003

M3 - Article

C2 - 15749458

AN - SCOPUS:14644399849

SN - 1043-6618

VL - 51

SP - 437

EP - 443

JO - Pharmacological Research

JF - Pharmacological Research

IS - 5

ER -

Induction of apoptosis and inhibition of telomerase activity by aqueous extract from Platycodon grandiflorum in human lung carcinoma cells

Abstract

Keywords

UN SDGs

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