Increasing angiogenic efficacy of conditioned medium using light stimulation of human adipose-derived stem cells

Yu Jin Kim; Sang Ho Lee; Jisoo Im; Jihun Song; Han Young Kim; Suk Ho Bhang

doi:10.1038/s42003-022-03838-3

Increasing angiogenic efficacy of conditioned medium using light stimulation of human adipose-derived stem cells

Yu Jin Kim
, Sang Ho Lee
, Jisoo Im
, Jihun Song
, Han Young Kim
, Suk Ho Bhang

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Conditioned medium (CM) contains various therapeutic molecules produced by cells. However, the low concentration of therapeutic molecules in CM is a major challenge for successful tissue regeneration. Here, we aim to develop a CM enriched in angiogenic paracrine factors for the treatment of ischemic diseases. Combining spheroidal culture and light irradiation significantly upregulates the angiogenic factor expression in human adipose-derived stem cells (hADSCs). Spheroids of light-irradiated hADSCs (SR group) show significantly enhanced expression of angiogenic paracrine factors compared with spheroids without light stimulation. Enhanced viability, migration, and angiogenesis are observed in cells treated with CM derived from the SR group. Furthermore, we performed in vivo experiments using a mouse hindlimb ischemia model; the results demonstrate that CM derived from densely cultured spheroids of light-irradiated hADSCs induced increased angiogenesis in vivo. In conclusion, our proposed approach of using light to stimulate stem cells may overcome the major drawbacks of CM-based therapies.

Original language	English
Article number	957
Journal	Communications Biology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/s42003-022-03838-3
State	Published - Dec 2022

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title = "Increasing angiogenic efficacy of conditioned medium using light stimulation of human adipose-derived stem cells",

abstract = "Conditioned medium (CM) contains various therapeutic molecules produced by cells. However, the low concentration of therapeutic molecules in CM is a major challenge for successful tissue regeneration. Here, we aim to develop a CM enriched in angiogenic paracrine factors for the treatment of ischemic diseases. Combining spheroidal culture and light irradiation significantly upregulates the angiogenic factor expression in human adipose-derived stem cells (hADSCs). Spheroids of light-irradiated hADSCs (SR group) show significantly enhanced expression of angiogenic paracrine factors compared with spheroids without light stimulation. Enhanced viability, migration, and angiogenesis are observed in cells treated with CM derived from the SR group. Furthermore, we performed in vivo experiments using a mouse hindlimb ischemia model; the results demonstrate that CM derived from densely cultured spheroids of light-irradiated hADSCs induced increased angiogenesis in vivo. In conclusion, our proposed approach of using light to stimulate stem cells may overcome the major drawbacks of CM-based therapies.",

author = "Kim, \{Yu Jin\} and Lee, \{Sang Ho\} and Jisoo Im and Jihun Song and Kim, \{Han Young\} and Bhang, \{Suk Ho\}",

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doi = "10.1038/s42003-022-03838-3",

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T1 - Increasing angiogenic efficacy of conditioned medium using light stimulation of human adipose-derived stem cells

AU - Kim, Yu Jin

AU - Lee, Sang Ho

AU - Im, Jisoo

AU - Song, Jihun

AU - Kim, Han Young

AU - Bhang, Suk Ho

PY - 2022/12

Y1 - 2022/12

N2 - Conditioned medium (CM) contains various therapeutic molecules produced by cells. However, the low concentration of therapeutic molecules in CM is a major challenge for successful tissue regeneration. Here, we aim to develop a CM enriched in angiogenic paracrine factors for the treatment of ischemic diseases. Combining spheroidal culture and light irradiation significantly upregulates the angiogenic factor expression in human adipose-derived stem cells (hADSCs). Spheroids of light-irradiated hADSCs (SR group) show significantly enhanced expression of angiogenic paracrine factors compared with spheroids without light stimulation. Enhanced viability, migration, and angiogenesis are observed in cells treated with CM derived from the SR group. Furthermore, we performed in vivo experiments using a mouse hindlimb ischemia model; the results demonstrate that CM derived from densely cultured spheroids of light-irradiated hADSCs induced increased angiogenesis in vivo. In conclusion, our proposed approach of using light to stimulate stem cells may overcome the major drawbacks of CM-based therapies.

AB - Conditioned medium (CM) contains various therapeutic molecules produced by cells. However, the low concentration of therapeutic molecules in CM is a major challenge for successful tissue regeneration. Here, we aim to develop a CM enriched in angiogenic paracrine factors for the treatment of ischemic diseases. Combining spheroidal culture and light irradiation significantly upregulates the angiogenic factor expression in human adipose-derived stem cells (hADSCs). Spheroids of light-irradiated hADSCs (SR group) show significantly enhanced expression of angiogenic paracrine factors compared with spheroids without light stimulation. Enhanced viability, migration, and angiogenesis are observed in cells treated with CM derived from the SR group. Furthermore, we performed in vivo experiments using a mouse hindlimb ischemia model; the results demonstrate that CM derived from densely cultured spheroids of light-irradiated hADSCs induced increased angiogenesis in vivo. In conclusion, our proposed approach of using light to stimulate stem cells may overcome the major drawbacks of CM-based therapies.

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DO - 10.1038/s42003-022-03838-3

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VL - 5

JO - Communications Biology

JF - Communications Biology

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Increasing angiogenic efficacy of conditioned medium using light stimulation of human adipose-derived stem cells

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