Increased cytoplasmic levels of CIS, SOCS1, SOCS2, or SOCS3 are required for nuclear translocation

Kyeong Hee Lee; Kyeong Jin Moon; Hyung Sik Kim; Byong Chul Yoo; Seoyoung Park; Ho Lee; Soim Kwon; Eun Sook Lee; Sungpil Yoon

doi:10.1016/j.febslet.2008.05.039

Increased cytoplasmic levels of CIS, SOCS1, SOCS2, or SOCS3 are required for nuclear translocation

Kyeong Hee Lee, Kyeong Jin Moon, Hyung Sik Kim, Byong Chul Yoo, Seoyoung Park, Ho Lee, Soim Kwon, Eun Sook Lee, Sungpil Yoon

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We investigated the cellular localization of ectopically-expressed CIS, SOCS1, SOCS2 and SOCS3 proteins. We found that SOCS proteins localize to the nucleus where they reduce Stat3 proteins and that the presence of proteasome inhibitors increased SOCS nuclear localization. Our results indicate that increased nuclear localization resulted from increased levels of SOCS proteins in the cytoplasm. Finally, we demonstrate that the same effect occurs with endogenously-expressed SOCS proteins. These observations suggest that increased cytoplasmic levels of proteins in the SOCS family are regulated through nuclear translocation.

Original language	English
Pages (from-to)	2319-2324
Number of pages	6
Journal	FEBS Letters
Volume	582
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2008.05.039
State	Published - 25 Jun 2008
Externally published	Yes

Keywords

Cytoplasm
Localization
Nuclei
Proteasome inhibitor
SOCS

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10.1016/j.febslet.2008.05.039

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title = "Increased cytoplasmic levels of CIS, SOCS1, SOCS2, or SOCS3 are required for nuclear translocation",

abstract = "We investigated the cellular localization of ectopically-expressed CIS, SOCS1, SOCS2 and SOCS3 proteins. We found that SOCS proteins localize to the nucleus where they reduce Stat3 proteins and that the presence of proteasome inhibitors increased SOCS nuclear localization. Our results indicate that increased nuclear localization resulted from increased levels of SOCS proteins in the cytoplasm. Finally, we demonstrate that the same effect occurs with endogenously-expressed SOCS proteins. These observations suggest that increased cytoplasmic levels of proteins in the SOCS family are regulated through nuclear translocation.",

keywords = "Cytoplasm, Localization, Nuclei, Proteasome inhibitor, SOCS",

author = "Lee, \{Kyeong Hee\} and Moon, \{Kyeong Jin\} and Kim, \{Hyung Sik\} and Yoo, \{Byong Chul\} and Seoyoung Park and Ho Lee and Soim Kwon and Lee, \{Eun Sook\} and Sungpil Yoon",

year = "2008",

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doi = "10.1016/j.febslet.2008.05.039",

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TY - JOUR

T1 - Increased cytoplasmic levels of CIS, SOCS1, SOCS2, or SOCS3 are required for nuclear translocation

AU - Lee, Kyeong Hee

AU - Moon, Kyeong Jin

AU - Kim, Hyung Sik

AU - Yoo, Byong Chul

AU - Park, Seoyoung

AU - Lee, Ho

AU - Kwon, Soim

AU - Lee, Eun Sook

AU - Yoon, Sungpil

PY - 2008/6/25

Y1 - 2008/6/25

N2 - We investigated the cellular localization of ectopically-expressed CIS, SOCS1, SOCS2 and SOCS3 proteins. We found that SOCS proteins localize to the nucleus where they reduce Stat3 proteins and that the presence of proteasome inhibitors increased SOCS nuclear localization. Our results indicate that increased nuclear localization resulted from increased levels of SOCS proteins in the cytoplasm. Finally, we demonstrate that the same effect occurs with endogenously-expressed SOCS proteins. These observations suggest that increased cytoplasmic levels of proteins in the SOCS family are regulated through nuclear translocation.

AB - We investigated the cellular localization of ectopically-expressed CIS, SOCS1, SOCS2 and SOCS3 proteins. We found that SOCS proteins localize to the nucleus where they reduce Stat3 proteins and that the presence of proteasome inhibitors increased SOCS nuclear localization. Our results indicate that increased nuclear localization resulted from increased levels of SOCS proteins in the cytoplasm. Finally, we demonstrate that the same effect occurs with endogenously-expressed SOCS proteins. These observations suggest that increased cytoplasmic levels of proteins in the SOCS family are regulated through nuclear translocation.

KW - Cytoplasm

KW - Localization

KW - Nuclei

KW - Proteasome inhibitor

KW - SOCS

UR - https://www.scopus.com/pages/publications/46549086459

U2 - 10.1016/j.febslet.2008.05.039

DO - 10.1016/j.febslet.2008.05.039

M3 - Article

C2 - 18538139

AN - SCOPUS:46549086459

SN - 0014-5793

VL - 582

SP - 2319

EP - 2324

JO - FEBS Letters

JF - FEBS Letters

IS - 15

ER -

Increased cytoplasmic levels of CIS, SOCS1, SOCS2, or SOCS3 are required for nuclear translocation

Abstract

Keywords

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