In vitro thrombin dose response on Madin Darby Canine Kidney cell monolayer

Rashid Amin; Imran Shakir; Ishrat Sultana; Sung Ha Park; Rafaqat Hussain

doi:10.1142/S1793292011002718

In vitro thrombin dose response on Madin Darby Canine Kidney cell monolayer

Rashid Amin
, Imran Shakir
, Ishrat Sultana
, Sung Ha Park
, Rafaqat Hussain

Research output: Contribution to journal › Article › peer-review

Abstract

Epithelial cells are known to play an important role in sustaining the airway barrier that may be impaired in certain inflammatory conditions. Recently, the use of thrombin has been reported to open the airway of patients with asthma as well as enhance the permeability of endothelial cell monolayers. We designed an in vitro model of Madin Darby Canine Kidney (MDCK) cells and the physiological functions of this model were evaluated by measuring the transepithelial resistance (TER). The epithelial cytoskeletal organization was observed by staining with Bisbenzimide and Rodamine-Phalloidin (BBZ-Phalloidin) and confirmed by fluorescence microscopy. Measurements of the TER generated values up to 2020 Ω/cm². A dose response of thrombin was observed, showing the permeability changes in the MDCK monolayer and subsequent recovery. A relationship between TER values and cytoskeletal organization was also observed and discussed.

Original language	English
Pages (from-to)	333-336
Number of pages	4
Journal	Nano
Volume	6
Issue number	4
DOIs	https://doi.org/10.1142/S1793292011002718
State	Published - Aug 2011
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

drug delivery
epithelial cell
Ion transport
membrane
permeability
transepithelial resistance

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10.1142/S1793292011002718

Cite this

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title = "In vitro thrombin dose response on Madin Darby Canine Kidney cell monolayer",

abstract = "Epithelial cells are known to play an important role in sustaining the airway barrier that may be impaired in certain inflammatory conditions. Recently, the use of thrombin has been reported to open the airway of patients with asthma as well as enhance the permeability of endothelial cell monolayers. We designed an in vitro model of Madin Darby Canine Kidney (MDCK) cells and the physiological functions of this model were evaluated by measuring the transepithelial resistance (TER). The epithelial cytoskeletal organization was observed by staining with Bisbenzimide and Rodamine-Phalloidin (BBZ-Phalloidin) and confirmed by fluorescence microscopy. Measurements of the TER generated values up to 2020 Ω/cm2. A dose response of thrombin was observed, showing the permeability changes in the MDCK monolayer and subsequent recovery. A relationship between TER values and cytoskeletal organization was also observed and discussed.",

keywords = "drug delivery, epithelial cell, Ion transport, membrane, permeability, transepithelial resistance",

author = "Rashid Amin and Imran Shakir and Ishrat Sultana and Park, \{Sung Ha\} and Rafaqat Hussain",

year = "2011",

month = aug,

doi = "10.1142/S1793292011002718",

language = "English",

volume = "6",

pages = "333--336",

journal = "Nano",

issn = "1793-2920",

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TY - JOUR

T1 - In vitro thrombin dose response on Madin Darby Canine Kidney cell monolayer

AU - Amin, Rashid

AU - Shakir, Imran

AU - Sultana, Ishrat

AU - Park, Sung Ha

AU - Hussain, Rafaqat

PY - 2011/8

Y1 - 2011/8

N2 - Epithelial cells are known to play an important role in sustaining the airway barrier that may be impaired in certain inflammatory conditions. Recently, the use of thrombin has been reported to open the airway of patients with asthma as well as enhance the permeability of endothelial cell monolayers. We designed an in vitro model of Madin Darby Canine Kidney (MDCK) cells and the physiological functions of this model were evaluated by measuring the transepithelial resistance (TER). The epithelial cytoskeletal organization was observed by staining with Bisbenzimide and Rodamine-Phalloidin (BBZ-Phalloidin) and confirmed by fluorescence microscopy. Measurements of the TER generated values up to 2020 Ω/cm2. A dose response of thrombin was observed, showing the permeability changes in the MDCK monolayer and subsequent recovery. A relationship between TER values and cytoskeletal organization was also observed and discussed.

AB - Epithelial cells are known to play an important role in sustaining the airway barrier that may be impaired in certain inflammatory conditions. Recently, the use of thrombin has been reported to open the airway of patients with asthma as well as enhance the permeability of endothelial cell monolayers. We designed an in vitro model of Madin Darby Canine Kidney (MDCK) cells and the physiological functions of this model were evaluated by measuring the transepithelial resistance (TER). The epithelial cytoskeletal organization was observed by staining with Bisbenzimide and Rodamine-Phalloidin (BBZ-Phalloidin) and confirmed by fluorescence microscopy. Measurements of the TER generated values up to 2020 Ω/cm2. A dose response of thrombin was observed, showing the permeability changes in the MDCK monolayer and subsequent recovery. A relationship between TER values and cytoskeletal organization was also observed and discussed.

KW - drug delivery

KW - epithelial cell

KW - Ion transport

KW - membrane

KW - permeability

KW - transepithelial resistance

UR - https://www.scopus.com/pages/publications/79961060275

U2 - 10.1142/S1793292011002718

DO - 10.1142/S1793292011002718

M3 - Article

AN - SCOPUS:79961060275

SN - 1793-2920

VL - 6

SP - 333

EP - 336

JO - Nano

JF - Nano

IS - 4

ER -

In vitro thrombin dose response on Madin Darby Canine Kidney cell monolayer

Abstract

UN SDGs

Keywords

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