In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin

Yu Ri Kang; Doo Ryeon Chung; Jungok Kim; Jin Yang Baek; So Hyun Kim; Young Eun Ha; Cheol In Kang; Kyong Ran Peck; Jae Hoon Song

doi:10.1016/j.diagmicrobio.2016.08.009

In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin

Yu Ri Kang
, Doo Ryeon Chung
, Jungok Kim
, Jin Yang Baek
, So Hyun Kim
, Young Eun Ha
, Cheol In Kang
, Kyong Ran Peck
, Jae Hoon Song

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The aim of our study was to determine the potential for synergistic effect of vancomycin combined with a non–beta-lactam agent and of combinations of orally available non-beta-lactam agents against vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). Three VISA isolates, three hVISA isolates, and two vancomycin-susceptible S. aureus (VSSA) isolates were used. The combinations included vancomycin and one of clindamycin, ciprofloxacin, gentamicin, rifampicin, or trimethoprim/sulfamethoxazole. Pairwise combinations among five non–beta-lactam agents were also tested for synergy. Synergy was determined using time-kill curves at 24 h. Vancomycin combined with either ciprofloxacin or gentamicin showed synergy against some isolates of VISA, hVISA and VSSA. Vancomycin combined with trimethoprim/sulfamethoxazole showed synergy against VISA and hVISA. Among orally available non-beta-lactam agents, only ciprofloxacin combined with either clindamycin or trimethoprim/sulfamethoxazole showed synergy against one isolate of VISA.

Original language	English
Pages (from-to)	293-299
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	86
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2016.08.009
State	Published - 1 Nov 2016

Keywords

Bactericidal activity
Drug resistance
Synergy
Time-kill study

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10.1016/j.diagmicrobio.2016.08.009

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Kang, Y. R., Chung, D. R., Kim, J., Baek, J. Y., Kim, S. H., Ha, Y. E., Kang, C. I., Peck, K. R., & Song, J. H. (2016). In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin. Diagnostic Microbiology and Infectious Disease, 86(3), 293-299. https://doi.org/10.1016/j.diagmicrobio.2016.08.009

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title = "In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin",

abstract = "The aim of our study was to determine the potential for synergistic effect of vancomycin combined with a non–beta-lactam agent and of combinations of orally available non-beta-lactam agents against vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). Three VISA isolates, three hVISA isolates, and two vancomycin-susceptible S. aureus (VSSA) isolates were used. The combinations included vancomycin and one of clindamycin, ciprofloxacin, gentamicin, rifampicin, or trimethoprim/sulfamethoxazole. Pairwise combinations among five non–beta-lactam agents were also tested for synergy. Synergy was determined using time-kill curves at 24 h. Vancomycin combined with either ciprofloxacin or gentamicin showed synergy against some isolates of VISA, hVISA and VSSA. Vancomycin combined with trimethoprim/sulfamethoxazole showed synergy against VISA and hVISA. Among orally available non-beta-lactam agents, only ciprofloxacin combined with either clindamycin or trimethoprim/sulfamethoxazole showed synergy against one isolate of VISA.",

keywords = "Bactericidal activity, Drug resistance, Synergy, Time-kill study",

author = "Kang, \{Yu Ri\} and Chung, \{Doo Ryeon\} and Jungok Kim and Baek, \{Jin Yang\} and Kim, \{So Hyun\} and Ha, \{Young Eun\} and Kang, \{Cheol In\} and Peck, \{Kyong Ran\} and Song, \{Jae Hoon\}",

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year = "2016",

month = nov,

day = "1",

doi = "10.1016/j.diagmicrobio.2016.08.009",

language = "English",

volume = "86",

pages = "293--299",

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Kang, YR, Chung, DR, Kim, J, Baek, JY, Kim, SH, Ha, YE, Kang, CI , Peck, KR & Song, JH 2016, 'In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin', Diagnostic Microbiology and Infectious Disease, vol. 86, no. 3, pp. 293-299. https://doi.org/10.1016/j.diagmicrobio.2016.08.009

In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin. / Kang, Yu Ri; Chung, Doo Ryeon; Kim, Jungok et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 86, No. 3, 01.11.2016, p. 293-299.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin

AU - Kang, Yu Ri

AU - Chung, Doo Ryeon

AU - Kim, Jungok

AU - Baek, Jin Yang

AU - Kim, So Hyun

AU - Ha, Young Eun

AU - Kang, Cheol In

AU - Peck, Kyong Ran

AU - Song, Jae Hoon

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The aim of our study was to determine the potential for synergistic effect of vancomycin combined with a non–beta-lactam agent and of combinations of orally available non-beta-lactam agents against vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). Three VISA isolates, three hVISA isolates, and two vancomycin-susceptible S. aureus (VSSA) isolates were used. The combinations included vancomycin and one of clindamycin, ciprofloxacin, gentamicin, rifampicin, or trimethoprim/sulfamethoxazole. Pairwise combinations among five non–beta-lactam agents were also tested for synergy. Synergy was determined using time-kill curves at 24 h. Vancomycin combined with either ciprofloxacin or gentamicin showed synergy against some isolates of VISA, hVISA and VSSA. Vancomycin combined with trimethoprim/sulfamethoxazole showed synergy against VISA and hVISA. Among orally available non-beta-lactam agents, only ciprofloxacin combined with either clindamycin or trimethoprim/sulfamethoxazole showed synergy against one isolate of VISA.

AB - The aim of our study was to determine the potential for synergistic effect of vancomycin combined with a non–beta-lactam agent and of combinations of orally available non-beta-lactam agents against vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). Three VISA isolates, three hVISA isolates, and two vancomycin-susceptible S. aureus (VSSA) isolates were used. The combinations included vancomycin and one of clindamycin, ciprofloxacin, gentamicin, rifampicin, or trimethoprim/sulfamethoxazole. Pairwise combinations among five non–beta-lactam agents were also tested for synergy. Synergy was determined using time-kill curves at 24 h. Vancomycin combined with either ciprofloxacin or gentamicin showed synergy against some isolates of VISA, hVISA and VSSA. Vancomycin combined with trimethoprim/sulfamethoxazole showed synergy against VISA and hVISA. Among orally available non-beta-lactam agents, only ciprofloxacin combined with either clindamycin or trimethoprim/sulfamethoxazole showed synergy against one isolate of VISA.

KW - Bactericidal activity

KW - Drug resistance

KW - Synergy

KW - Time-kill study

UR - https://www.scopus.com/pages/publications/84992091726

U2 - 10.1016/j.diagmicrobio.2016.08.009

DO - 10.1016/j.diagmicrobio.2016.08.009

M3 - Article

C2 - 27591174

AN - SCOPUS:84992091726

SN - 0732-8893

VL - 86

SP - 293

EP - 299

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

In vitro synergistic effects of various combinations of vancomycin and non-beta-lactams against Staphylococcus aureus with reduced susceptibility to vancomycin

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Keywords

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