In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

Rong Huang; Abdul Rahim Ferhan; Longhua Guo; Bin Qiu; Zhenyu Lin; Dong Hwan Kim; Guonan Chen

doi:10.1039/c3ra47012a

In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

Rong Huang
, Abdul Rahim Ferhan
, Longhua Guo
, Bin Qiu
, Zhenyu Lin
, Dong Hwan Kim
, Guonan Chen

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.

Original language	English
Pages (from-to)	4883-4888
Number of pages	6
Journal	RSC Advances
Volume	4
Issue number	10
DOIs	https://doi.org/10.1039/c3ra47012a
State	Published - 2014
Externally published	Yes

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10.1039/c3ra47012a

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title = "In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation",

abstract = "We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.",

author = "Rong Huang and Ferhan, \{Abdul Rahim\} and Longhua Guo and Bin Qiu and Zhenyu Lin and Kim, \{Dong Hwan\} and Guonan Chen",

year = "2014",

doi = "10.1039/c3ra47012a",

language = "English",

volume = "4",

pages = "4883--4888",

journal = "RSC Advances",

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TY - JOUR

T1 - In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

AU - Huang, Rong

AU - Ferhan, Abdul Rahim

AU - Guo, Longhua

AU - Qiu, Bin

AU - Lin, Zhenyu

AU - Kim, Dong Hwan

AU - Chen, Guonan

PY - 2014

Y1 - 2014

N2 - We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.

AB - We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.

UR - https://www.scopus.com/pages/publications/84891774483

U2 - 10.1039/c3ra47012a

DO - 10.1039/c3ra47012a

M3 - Article

AN - SCOPUS:84891774483

SN - 2046-2069

VL - 4

SP - 4883

EP - 4888

JO - RSC Advances

JF - RSC Advances

IS - 10

ER -

In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

Abstract

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