Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis

You Jin Lee; Sun Young Park; Sun Gun Kim; Da Jung Park; Jum Soon Kang; Sang Joon Lee; Sik Yoon; Young Hun Kim; Yoe Sik Bae; Young Whan Choi

doi:10.1016/j.bbrc.2009.12.131

Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis

You Jin Lee, Sun Young Park, Sun Gun Kim, Da Jung Park, Jum Soon Kang, Sang Joon Lee, Sik Yoon, Young Hun Kim, Yoe Sik Bae, Young Whan Choi

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A novel α-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. α-iso-cubebenol inhibited LPS-induced nitric oxide (NO) and prostaglandin E₂ (PGE₂) production. Consistent with these findings, α-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. α-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-κB p65 subunit. Furthermore, α-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel α-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin.

Original language	English
Pages (from-to)	1687-1692
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	391
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2009.12.131
State	Published - 22 Jan 2010
Externally published	Yes

Keywords

α-iso-cubebenol
COX-2
iNOS
LPS
Macrophage
NF-κB

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10.1016/j.bbrc.2009.12.131

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Lee, Y. J., Park, S. Y., Kim, S. G., Park, D. J., Kang, J. S., Lee, S. J., Yoon, S., Kim, Y. H., Bae, Y. S., & Choi, Y. W. (2010). Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis. Biochemical and Biophysical Research Communications, 391(4), 1687-1692. https://doi.org/10.1016/j.bbrc.2009.12.131

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title = "Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis",

abstract = "A novel α-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. α-iso-cubebenol inhibited LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production. Consistent with these findings, α-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. α-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-κB p65 subunit. Furthermore, α-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel α-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin.",

keywords = "α-iso-cubebenol, COX-2, iNOS, LPS, Macrophage, NF-κB",

author = "Lee, \{You Jin\} and Park, \{Sun Young\} and Kim, \{Sun Gun\} and Park, \{Da Jung\} and Kang, \{Jum Soon\} and Lee, \{Sang Joon\} and Sik Yoon and Kim, \{Young Hun\} and Bae, \{Yoe Sik\} and Choi, \{Young Whan\}",

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doi = "10.1016/j.bbrc.2009.12.131",

language = "English",

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T1 - Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis

AU - Lee, You Jin

AU - Park, Sun Young

AU - Kim, Sun Gun

AU - Park, Da Jung

AU - Kang, Jum Soon

AU - Lee, Sang Joon

AU - Yoon, Sik

AU - Kim, Young Hun

AU - Bae, Yoe Sik

AU - Choi, Young Whan

PY - 2010/1/22

Y1 - 2010/1/22

N2 - A novel α-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. α-iso-cubebenol inhibited LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production. Consistent with these findings, α-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. α-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-κB p65 subunit. Furthermore, α-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel α-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin.

AB - A novel α-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. α-iso-cubebenol inhibited LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production. Consistent with these findings, α-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. α-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-κB p65 subunit. Furthermore, α-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel α-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin.

KW - α-iso-cubebenol

KW - COX-2

KW - iNOS

KW - LPS

KW - Macrophage

KW - NF-κB

UR - https://www.scopus.com/pages/publications/74449085913

U2 - 10.1016/j.bbrc.2009.12.131

DO - 10.1016/j.bbrc.2009.12.131

M3 - Article

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AN - SCOPUS:74449085913

SN - 0006-291X

VL - 391

SP - 1687

EP - 1692

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis

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