TY - JOUR
T1 - Hypertensive left ventricular hypertrophy
T2 - Relation to β-adrenergic receptor kinase-1 (βARK1) in peripheral lymphocytes
AU - Park, Sung Ji
AU - Choi, Dong Ju
AU - Kim, Choong Won
PY - 2004/5
Y1 - 2004/5
N2 - Background: Left ventricular hypertrophy (LVH) is associated with increased cardiovascular risk and altered sympathetic regulation in hypertension. Objectives: To determine whether the level of β-adrenergic receptor kinase-1 (βARK1) in lymphocytes is related to LVH in patients with hypertension. Methods: Forty-nine patients with untreated essential hypertension were recruited to the study and classified into two groups: left ventricular hypertrophy (LVH: left ventricular mass index ≥ 134 g/m2 in men and ≥ 110 g/ m2 in women; ages 52.4 ± 12.8 years, n = 25) and non-LVH (NLVH: left ventricular mass index < 134 g/m2 in men and < 110 g/m2 in women; ages 50.8 ± 13.1 years, n = 24). Lymphocytes were isolated from patients and quantitative-competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to estimate the expression of βARK1 in the lymphocytes. G-protein-coupled receptor kinase activity was assessed by rhodopsin phosphorylation assay. Results: The expression of βARK1 in lymphocytes was greater in the LVH group than in the NLVH group (0.0069 ± 0.002 ng compared with 0.0048 ± 0.0017 ng, P < 0.01) and correlated well with left ventricular mass index (r = 0.527, P < 0.001) and relative wall thickness (r = 0.627, P < 0.001). The concentration of βARK1 protein in lymphocytes from individuals with LVH was increased two-fold compared with that in the NLVM group (both n = 7). Lymphocyte G-protein-coupled receptor kinase activity from LVH was enhanced 1.7-fold compared with NLVH (1.03 ± 2.16 and 1.79 ± 1.87 pmol phosphate/min per mg protein, respectively; P < 0.05, n = 7 for each group). Conclusions: The concentration of βARK1 in lymphocytes is greater in hypertensive individuals with LVH than in those without LVH and parallels the degree of hypertrophy. Generalized alterations in β-adrenergic signalling, including βARK1, could be a major contributory factor in the development of LVH in hypertension, and the concentration of βARK1 in lymphocytes can reflect the development of LVH in a patient with hypertension.
AB - Background: Left ventricular hypertrophy (LVH) is associated with increased cardiovascular risk and altered sympathetic regulation in hypertension. Objectives: To determine whether the level of β-adrenergic receptor kinase-1 (βARK1) in lymphocytes is related to LVH in patients with hypertension. Methods: Forty-nine patients with untreated essential hypertension were recruited to the study and classified into two groups: left ventricular hypertrophy (LVH: left ventricular mass index ≥ 134 g/m2 in men and ≥ 110 g/ m2 in women; ages 52.4 ± 12.8 years, n = 25) and non-LVH (NLVH: left ventricular mass index < 134 g/m2 in men and < 110 g/m2 in women; ages 50.8 ± 13.1 years, n = 24). Lymphocytes were isolated from patients and quantitative-competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to estimate the expression of βARK1 in the lymphocytes. G-protein-coupled receptor kinase activity was assessed by rhodopsin phosphorylation assay. Results: The expression of βARK1 in lymphocytes was greater in the LVH group than in the NLVH group (0.0069 ± 0.002 ng compared with 0.0048 ± 0.0017 ng, P < 0.01) and correlated well with left ventricular mass index (r = 0.527, P < 0.001) and relative wall thickness (r = 0.627, P < 0.001). The concentration of βARK1 protein in lymphocytes from individuals with LVH was increased two-fold compared with that in the NLVM group (both n = 7). Lymphocyte G-protein-coupled receptor kinase activity from LVH was enhanced 1.7-fold compared with NLVH (1.03 ± 2.16 and 1.79 ± 1.87 pmol phosphate/min per mg protein, respectively; P < 0.05, n = 7 for each group). Conclusions: The concentration of βARK1 in lymphocytes is greater in hypertensive individuals with LVH than in those without LVH and parallels the degree of hypertrophy. Generalized alterations in β-adrenergic signalling, including βARK1, could be a major contributory factor in the development of LVH in hypertension, and the concentration of βARK1 in lymphocytes can reflect the development of LVH in a patient with hypertension.
KW - Cardiac hypertrophy
KW - Hypertension
KW - Lymphocyte
KW - β-adrenergic receptor kinase-1
UR - https://www.scopus.com/pages/publications/2542495281
U2 - 10.1097/00004872-200405000-00026
DO - 10.1097/00004872-200405000-00026
M3 - Article
C2 - 15097244
AN - SCOPUS:2542495281
SN - 0263-6352
VL - 22
SP - 1025
EP - 1032
JO - Journal of Hypertension
JF - Journal of Hypertension
IS - 5
ER -