Humicoccus flavidus gen. nov., sp. nov., isolated from soil

A Gram-positive, non-motile, spherical, non-spore-forming bacteria] strain, DS-52^T, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. It grew optimally at 25 °C and pH 6.0-7.0. Strain DS-52^T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and galactose, mannose, xylose and rhamnose as whole-cell sugars. It contained MK-8(H₄) and MK-9(H₄) as the predominant menaquinones and anteiso-C_15:0, iso-_C15:0 and C_17:0 as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidyldimethylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-52^T is most closely related to the genus Nakamurella of the suborder Frankineae. Strain DS-52^T exhibited 16S rRNA gene sequence similarity values of 96.5 % to Nakamurella multipartita JCM 9543^T and 92.0-93.9% to other members of the suborder Frankineae. The diagnostic diamino acid type and polar lipid profile of strain DS-52^T were the same as those of the genus Nakamurella. However, strain DS-52^T could be clearly distinguished from the genus Nakamurella by differences in predominant menaquinones, major fatty acids and cell-wall sugars. Accordingly, based on combined phenotypic, chernotaxonomic and phylogenetic data, strain DS-52^T (=KCTC 19127^T=CIP 108919^T) is proposed as the type strain of a novel species in a new genus, Humicoccus flavidus gen. nov., sp. nov.