Histone chaperones regulate histone exchange during transcription

Hye Jin Kim; Ja Hwan Seol; Jeung Whan Han; Hong Duk Youn; Eun Jung Cho

doi:10.1038/sj.emboj.7601870

Histone chaperones regulate histone exchange during transcription

Hye Jin Kim, Ja Hwan Seol, Jeung Whan Han, Hong Duk Youn, Eun Jung Cho

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.

Original language	English
Pages (from-to)	4467-4474
Number of pages	8
Journal	EMBO Journal
Volume	26
Issue number	21
DOIs	https://doi.org/10.1038/sj.emboj.7601870
State	Published - 31 Oct 2007

Keywords

Asf1
HIR
Histone exchange
Histone H3/H4 chaperone
Histone mobility

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10.1038/sj.emboj.7601870

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abstract = "Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.",

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AU - Kim, Hye Jin

AU - Seol, Ja Hwan

AU - Han, Jeung Whan

AU - Youn, Hong Duk

AU - Cho, Eun Jung

PY - 2007/10/31

Y1 - 2007/10/31

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AB - Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.

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Histone chaperones regulate histone exchange during transcription

Abstract

Keywords

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