Abstract
A label-free electrochemical immunosensor for the detection of neutrophil gelatinase-associated lipocalin (NGAL) is developed by the immobilization of rabbit polygonal lipocalin-2 antibody on gold nanoparticles attached on generation-1polyamidoamine (PAMAM) dendrimer (LA2/AuNPs/PAMAM)-modified gold electrode. The modification procedure was characterized by UV-vis, surface enhanced Raman spectroscopy and field-emission scanning electron microscopy techniques. The detection of NGAL is based on the enhancement of oxidation current on the modified electrodes upon the antigen-antibody interaction. The electrochemical immunosensor exhibited high sensitivity (1ngmL -1 (280pM) based on the signal-to-noise ratio 3), wide linear range (50-250ngmL -1) and long-term stability. The reliability of the developed immunosensor was investigated by the detection of NGAL in both blood serum and urine samples.
| Original language | English |
|---|---|
| Pages (from-to) | 32-36 |
| Number of pages | 5 |
| Journal | Biosensors and Bioelectronics |
| Volume | 31 |
| Issue number | 1 |
| DOIs | |
| State | Published - 15 Jan 2012 |
| Externally published | Yes |
Keywords
- AuNPs self-assembly
- Immunosensor
- NGAL, Acute kidney injury
- PAMAM
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