Highly efficient RNA-guided base editing in mouse embryos

Kyoungmi Kim; Seuk Min Ryu; Sang Tae Kim; Gayoung Baek; Daesik Kim; Kayeong Lim; Eugene Chung; Sunghyun Kim; Jin Soo Kim

doi:10.1038/nbt.3816

Highly efficient RNA-guided base editing in mouse embryos

Kyoungmi Kim
, Seuk Min Ryu
, Sang Tae Kim
, Gayoung Baek
, Daesik Kim
, Kayeong Lim
, Eugene Chung
, Sunghyun Kim
, Jin Soo Kim

Institute for Basic Science
Seoul National University

Research output: Contribution to journal › Article › peer-review

356 Scopus citations

Abstract

Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

Original language	English
Pages (from-to)	435-437
Number of pages	3
Journal	Nature Biotechnology
Volume	35
Issue number	5
DOIs	https://doi.org/10.1038/nbt.3816
State	Published - 1 May 2017
Externally published	Yes

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10.1038/nbt.3816

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abstract = "Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57\% and allelic frequencies of up to 100\%, demonstrating an efficient method to generate mice with targeted point mutations.",

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AU - Ryu, Seuk Min

AU - Kim, Sang Tae

AU - Baek, Gayoung

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AU - Lim, Kayeong

AU - Chung, Eugene

AU - Kim, Sunghyun

AU - Kim, Jin Soo

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AB - Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

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Highly efficient RNA-guided base editing in mouse embryos

Abstract

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