Haptotactic gradients for directed cell migration: Stimulation and inhibition using soluble factors

Jason T. Smith; Donghwan H. Kim; William M. Reichert

doi:10.2174/138620709788681871

Haptotactic gradients for directed cell migration: Stimulation and inhibition using soluble factors

Jason T. Smith, Donghwan H. Kim, William M. Reichert

Duke University

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migrationinhibiting LY294002 eliminated the directional component of cell migration at a 5 μM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.

Original language	English
Pages (from-to)	598-603
Number of pages	6
Journal	Combinatorial Chemistry and High Throughput Screening
Volume	12
Issue number	6
DOIs	https://doi.org/10.2174/138620709788681871
State	Published - Jul 2009
Externally published	Yes

Keywords

Acidic fibroblast growth factor
aFGF
Cell migration
Fibronectin
Gradient
Haptotaxis
LY294002
Microvascular endothelial cell
Morphology

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title = "Haptotactic gradients for directed cell migration: Stimulation and inhibition using soluble factors",

abstract = "A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migrationinhibiting LY294002 eliminated the directional component of cell migration at a 5 μM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.",

keywords = "Acidic fibroblast growth factor, aFGF, Cell migration, Fibronectin, Gradient, Haptotaxis, LY294002, Microvascular endothelial cell, Morphology",

author = "Smith, \{Jason T.\} and Kim, \{Donghwan H.\} and Reichert, \{William M.\}",

year = "2009",

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language = "English",

volume = "12",

pages = "598--603",

journal = "Combinatorial Chemistry and High Throughput Screening",

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T1 - Haptotactic gradients for directed cell migration

T2 - Stimulation and inhibition using soluble factors

AU - Smith, Jason T.

AU - Kim, Donghwan H.

AU - Reichert, William M.

PY - 2009/7

Y1 - 2009/7

N2 - A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migrationinhibiting LY294002 eliminated the directional component of cell migration at a 5 μM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.

AB - A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migrationinhibiting LY294002 eliminated the directional component of cell migration at a 5 μM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.

KW - Acidic fibroblast growth factor

KW - aFGF

KW - Cell migration

KW - Fibronectin

KW - Gradient

KW - Haptotaxis

KW - LY294002

KW - Microvascular endothelial cell

KW - Morphology

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U2 - 10.2174/138620709788681871

DO - 10.2174/138620709788681871

M3 - Article

C2 - 19601757

AN - SCOPUS:67650878999

SN - 1386-2073

VL - 12

SP - 598

EP - 603

JO - Combinatorial Chemistry and High Throughput Screening

JF - Combinatorial Chemistry and High Throughput Screening

IS - 6

ER -

Haptotactic gradients for directed cell migration: Stimulation and inhibition using soluble factors

Abstract

Keywords

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