Halobacillus campisalis sp. nov., containing mesodiaminopimelic acid in the cell-wall peptidoglycan and emended description of the genus Halobacillus

A Gram-positive or variable, motile and coccoid or oval-shaped bacterial strain, ASL-17^T, was isolated from a marine solar saltern of the Yellow Sea in Korea and its exact taxonomic position was investigated by a polyphasic approach. Strain ASL-17^T grew optimally at pH 7.0-8.0 and 37 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ASL-17^T is most closely affiliated phylogenetically to the genus Halobacillus. Strain ASL-17^T exhibited 16S rRNA gene sequence similarity values of 97.7-98.6% to the type strains of recognized Halobacillus species. Interestingly, strain ASL-17^T had cell-wall peptidoglycan based on meso-diaminopimelic acid, unlike other Halobacillus species. It contained MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C_15:0 anteiso-C_17:0 and iso-C_16:0. The DNA G+C content was 42.1 mol%. DNA-DNA relatedness data and differential phenotypic properties demonstrated that strain ASL-17^T can be differentiated from recognized Halobacillus species. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain ASL-17^T represents a novel species of the genus Halobacillus, for which the name Halobacillus campisalis sp. nov. is proposed. The type strain is ASL-17^T (=KCTC 13144^T =CCUG 54360^T).