Gryllotalpicola soli sp. nov., isolated from soil

A novel Gram-stain-positive, short rod-shaped, non-flagellated and mesophilic strain, KIS12-7^T, isolated from a soil sample collected from Daecheong-Island in Ongjin County, Republic of Korea, was studied using a polyphasic approach. Phylogenetic trees based on the 16S rRNA gene sequence revealed that the novel strain was a member of the genus Gryllotalpicola, showing more than 97.0 % sequence similarity with Gryllotalpicola daejeonensis RU-04^T (98.0 %), Gryllotalpicola koreensis RU-16^T (97.7 %) andGryllotalpicola kribbensis PU-02^T (97.3 %). However, DNA–DNA relatedness values demonstrated that strain KIS12-7^T could be clearly distinguished from closely related species of the genus Gryllotalpicola. The cell-wall peptidoglycan of strain KIS12-7^T was of the type B2 and the acyl type was acetyl. The predominant menaquinones were MK-11 and MK-10. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The G+C content of the genomic DNA was 72.1 mol%. On the basis of the evidence presented, strain KIS12-7^T is a representative of a novel species of the genus Gryllotalpicola, and the name Gryllotalpicola solisp. nov. is proposed; the type strain is KIS12-7^T (= DSM 27182^T = KACC 17302^T = NBRC 109659^T).