Gordonia hankookensis sp. nov., isolated from soil

A Gram-positive, aerobic and non-motile bacterial strain, designated ON-33^T, was subjected to a study based on a polyphasic approach to determine its exact taxonomic position. Strain ON-33^T grew optimally at pH 7.0-7.5 and 30 °C. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ON-33^T fell within the clade comprising Gordonia species, clustering with Gordonia soli CC-AB07^T, with which it exhibited 16S rRNA gene sequence similarity of 98.5 %. The chemotaxonomic properties of strain ON-33^T were consistent with those shared by members of the genus Gordonia. The peptidoglycan type was based on meso-diaminopimelic acid and the whole-cell sugars were arabinose and galactose. The predominant menaquinone was MK-9(H₂). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were C_16:0, iso-C_15:0 2-OH and/or C_16:1ω7c, 10-methyl C_18:0 and C_18:1ω9c. The DNA G+C content was 66.9 mol%. Strain ON-33^T exhibited a DNA- DNA relatedness value of 13 % to G. soli DSM 44995^T and could be differentiated from G. soli by differences in phenotypic characteristics. On the basis of the data obtained, strain ON-33^T is considered to represent a novel species of the genus Gordonia, for which the name Gordonia hankookensis sp. nov., is proposed. The type strain is ON-33^T (=KCTC 19599^T=CCUG 57507^T).