Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis

Jeong Ran Kim; Bong Seok Kang; Jeong Heon Ko; Jin Suk Park; Sang Jae Kim; Gil Hwan Bai; Tae Ho Chung; Kyung Soo Nam; Yong Kyung Choi; In Sung Choe; Tae Wha Chung; Young Choon Lee; Cheorl Ho Kim

Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis

Jeong Ran Kim
, Bong Seok Kang
, Jeong Heon Ko
, Jin Suk Park
, Sang Jae Kim
, Gil Hwan Bai
, Tae Ho Chung
, Kyung Soo Nam
, Yong Kyung Choi
, In Sung Choe
, Tae Wha Chung
, Young Choon Lee
, Cheorl Ho Kim

Dongguk University
Hannam University
Korea Research Institute of Bioscience and Biotechnology
The Korean National Tuberculosis Association
Kyungpook National University

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.

Original language	English
Pages (from-to)	569-573
Number of pages	5
Journal	Journal of Biochemistry and Molecular Biology
Volume	29
Issue number	6
State	Published - 30 Nov 1996
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Genome
Mycobacteria
Pulsed-fielded gel electrophoresis

Cite this

Kim, J. R., Kang, B. S., Ko, J. H., Park, J. S., Kim, S. J., Bai, G. H., Chung, T. H., Nam, K. S., Choi, Y. K., Choe, I. S., Chung, T. W., Lee, Y. C., & Kim, C. H. (1996). Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis. Journal of Biochemistry and Molecular Biology, 29(6), 569-573.

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title = "Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis",

abstract = "Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.",

keywords = "Genome, Mycobacteria, Pulsed-fielded gel electrophoresis",

author = "Kim, \{Jeong Ran\} and Kang, \{Bong Seok\} and Ko, \{Jeong Heon\} and Park, \{Jin Suk\} and Kim, \{Sang Jae\} and Bai, \{Gil Hwan\} and Chung, \{Tae Ho\} and Nam, \{Kyung Soo\} and Choi, \{Yong Kyung\} and Choe, \{In Sung\} and Chung, \{Tae Wha\} and Lee, \{Young Choon\} and Kim, \{Cheorl Ho\}",

year = "1996",

month = nov,

day = "30",

language = "English",

volume = "29",

pages = "569--573",

journal = "Journal of Biochemistry and Molecular Biology",

issn = "1225-8687",

publisher = "Springer Verlag",

number = "6",

}

Kim, JR, Kang, BS, Ko, JH, Park, JS, Kim, SJ, Bai, GH, Chung, TH, Nam, KS, Choi, YK, Choe, IS, Chung, TW, Lee, YC & Kim, CH 1996, 'Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis', Journal of Biochemistry and Molecular Biology, vol. 29, no. 6, pp. 569-573.

Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis. / Kim, Jeong Ran; Kang, Bong Seok; Ko, Jeong Heon et al.
In: Journal of Biochemistry and Molecular Biology, Vol. 29, No. 6, 30.11.1996, p. 569-573.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis

AU - Kim, Jeong Ran

AU - Kang, Bong Seok

AU - Ko, Jeong Heon

AU - Park, Jin Suk

AU - Kim, Sang Jae

AU - Bai, Gil Hwan

AU - Chung, Tae Ho

AU - Nam, Kyung Soo

AU - Choi, Yong Kyung

AU - Choe, In Sung

AU - Chung, Tae Wha

AU - Lee, Young Choon

AU - Kim, Cheorl Ho

PY - 1996/11/30

Y1 - 1996/11/30

N2 - Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.

AB - Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.

KW - Genome

KW - Mycobacteria

KW - Pulsed-fielded gel electrophoresis

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M3 - Article

AN - SCOPUS:0030356643

SN - 1225-8687

VL - 29

SP - 569

EP - 573

JO - Journal of Biochemistry and Molecular Biology

JF - Journal of Biochemistry and Molecular Biology

IS - 6

ER -

Genomic heterogeneity in clinical strains of mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis

Abstract

UN SDGs

Keywords

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