Flavonoids inhibit the AU-rich element binding of HuC

Hojoong Kwak; Kyung Chae Jeong; Min Ju Chae; Soo Youl Kim; Woong Yang Park

doi:10.5483/BMBRep.2009.42.1.041

Flavonoids inhibit the AU-rich element binding of HuC

Hojoong Kwak
, Kyung Chae Jeong
, Min Ju Chae
, Soo Youl Kim
, Woong Yang Park

Seoul National University
National Cancer Center Korea

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC₅₀ values were low (0.2-1.8 μM).

Original language	English
Pages (from-to)	41-46
Number of pages	6
Journal	BMB Reports
Volume	42
Issue number	1
DOIs	https://doi.org/10.5483/BMBRep.2009.42.1.041
State	Published - Jan 2009
Externally published	Yes

Keywords

AU-rich element
Electrophoretic mobility shift assay
Flavonoids
HuC
Natural compounds

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10.5483/BMBRep.2009.42.1.041

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title = "Flavonoids inhibit the AU-rich element binding of HuC",

abstract = "Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC50 values were low (0.2-1.8 μM).",

keywords = "AU-rich element, Electrophoretic mobility shift assay, Flavonoids, HuC, Natural compounds",

author = "Hojoong Kwak and Jeong, \{Kyung Chae\} and Chae, \{Min Ju\} and Kim, \{Soo Youl\} and Park, \{Woong Yang\}",

year = "2009",

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language = "English",

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AU - Chae, Min Ju

AU - Kim, Soo Youl

AU - Park, Woong Yang

PY - 2009/1

Y1 - 2009/1

N2 - Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC50 values were low (0.2-1.8 μM).

AB - Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC50 values were low (0.2-1.8 μM).

KW - AU-rich element

KW - Electrophoretic mobility shift assay

KW - Flavonoids

KW - HuC

KW - Natural compounds

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ER -

Flavonoids inhibit the AU-rich element binding of HuC

Abstract

Keywords

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