Fast on-fiber derivatization and GC/MS analysis of phytohormones in wheat based on pencil-type coated carbon fibers

  • Yilin Zou
  • , Long Yue Meng
  • , Meiyu Cui
  • , Jinhua Zhao
  • , Miao He
  • , Jiman Kim
  • , Maurizio Quinto
  • , Hai Bo Shang
  • , Donghao Li

Research output: Contribution to journalArticlepeer-review

Abstract

New coated carbon fibers (CCFs) have been synthesized, characterized and used as solid phase microextraction (SPME) matrix for the analysis of phytohormones (jasmonic acid, indole-3-acetic acid, and abscisic acid) in wheat samples. The SPME device, realized inserting CCFs in a pencil-type device, when coupled with gas chromatography-mass spectrometry, provides in few steps high recovery values (79–112%), fast on-fiber derivatization (30 s), good method reproducibility (RSD < 20%), low detection limits (0.5–2.1 ng g−1). The pencil-type CCFs-SPME device was successfully employed for the determination of phytohormone in wheat samples, allowing simple and quick extraction/derivatization/injection processes. The proposed device can be then considered as a promising and functional tool for fast and reliable extraction and preconcentration of analytes from real samples, allowing a simple derivatization procedure and direct injection in the chromatographic instrumentation.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalFood Chemistry
Volume274
DOIs
StatePublished - 15 Feb 2019
Externally publishedYes

Keywords

  • Coated carbon fibers
  • N,O-bis(trimethylsilyl)trifluoroacetamide
  • On-fiber derivatization
  • Phytohormones
  • Solid phase microextraction
  • Wheat

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