Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

Mi Kyoung Kim; Do Sik Min; Yoon Jeong Park; Jae Ho Kim; Sung Ho Ryu; Yoe Sik Bae

doi:10.1016/j.febslet.2007.03.078

Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

Mi Kyoung Kim, Do Sik Min, Yoon Jeong Park, Jae Ho Kim, Sung Ho Ryu, Yoe Sik Bae

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by G_i proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of G_i proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.

Original language	English
Pages (from-to)	1917-1922
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2007.03.078
State	Published - 1 May 2007
Externally published	Yes

Keywords

Annexin-1
Chemotaxis
fMLF
Formyl peptide receptor
Mesenchymal stem cells
Pertussis toxin-sensitive G-protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.febslet.2007.03.078

Cite this

@article{03828a0440fd43279cccb641d6400155,

title = "Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells",

abstract = "We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by Gi proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of Gi proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.",

keywords = "Annexin-1, Chemotaxis, fMLF, Formyl peptide receptor, Mesenchymal stem cells, Pertussis toxin-sensitive G-protein",

author = "Kim, \{Mi Kyoung\} and Min, \{Do Sik\} and Park, \{Yoon Jeong\} and Kim, \{Jae Ho\} and Ryu, \{Sung Ho\} and Bae, \{Yoe Sik\}",

year = "2007",

month = may,

day = "1",

doi = "10.1016/j.febslet.2007.03.078",

language = "English",

volume = "581",

pages = "1917--1922",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "9",

}

TY - JOUR

T1 - Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

AU - Kim, Mi Kyoung

AU - Min, Do Sik

AU - Park, Yoon Jeong

AU - Kim, Jae Ho

AU - Ryu, Sung Ho

AU - Bae, Yoe Sik

PY - 2007/5/1

Y1 - 2007/5/1

N2 - We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by Gi proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of Gi proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.

AB - We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by Gi proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of Gi proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.

KW - Annexin-1

KW - Chemotaxis

KW - fMLF

KW - Formyl peptide receptor

KW - Mesenchymal stem cells

KW - Pertussis toxin-sensitive G-protein

UR - https://www.scopus.com/pages/publications/34247141961

U2 - 10.1016/j.febslet.2007.03.078

DO - 10.1016/j.febslet.2007.03.078

M3 - Article

C2 - 17442310

AN - SCOPUS:34247141961

SN - 0014-5793

VL - 581

SP - 1917

EP - 1922

JO - FEBS Letters

JF - FEBS Letters

IS - 9

ER -

Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this