TY - JOUR
T1 - Experimental model of non-rhegmatogenous retinal detachment by laser photodynamic vascular thrombosis in rats
AU - Ham, D. I.
AU - Lee, J. G.
AU - Chung, H.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose. To fascilitate further investigation of non-rhegmatogenous retinal detachment, we developed a reproducible animal model of non-rhegmatogenous total retinal detachment in rats. Methods. 10 Sprague-Dawley albino rats received 40mg/kg of rose-bengal photosensitive dye intravenously after anesthesia. One minute later, occlusions of all the major retinal veins and underlying choriocapillaris were accomplished by argon-green laser photocoagulation (514.5nm in wave-length), using laser parameters of 0.1sec, 50μm, 150mW, and about 20 impulses per vessel. 10 eyes of 10 rats were treated, and the fluorescein angiography was performed immediately. Results. Fluorescein angiography showed complete occlusions of all the major retinal veins and underlying areas of choroidal non-perfusion. The retinal veins became dilated and tortuous and retinal edema developed in all eyes. One day after treatment, total retinal detachment, and intraretinal hemorrhage developed in all eyes. Vitreous hemorrhage occurred in 2/10 eyes. Conclusions. This is a reproducible model of non-rhegmatogenous retinal detachment in rats, using modified laser parameters. This will allow further investigation of pathophysiology of non-rhegmatogenous retinal detachment associated retinal and choroidal ischemia.
AB - Purpose. To fascilitate further investigation of non-rhegmatogenous retinal detachment, we developed a reproducible animal model of non-rhegmatogenous total retinal detachment in rats. Methods. 10 Sprague-Dawley albino rats received 40mg/kg of rose-bengal photosensitive dye intravenously after anesthesia. One minute later, occlusions of all the major retinal veins and underlying choriocapillaris were accomplished by argon-green laser photocoagulation (514.5nm in wave-length), using laser parameters of 0.1sec, 50μm, 150mW, and about 20 impulses per vessel. 10 eyes of 10 rats were treated, and the fluorescein angiography was performed immediately. Results. Fluorescein angiography showed complete occlusions of all the major retinal veins and underlying areas of choroidal non-perfusion. The retinal veins became dilated and tortuous and retinal edema developed in all eyes. One day after treatment, total retinal detachment, and intraretinal hemorrhage developed in all eyes. Vitreous hemorrhage occurred in 2/10 eyes. Conclusions. This is a reproducible model of non-rhegmatogenous retinal detachment in rats, using modified laser parameters. This will allow further investigation of pathophysiology of non-rhegmatogenous retinal detachment associated retinal and choroidal ischemia.
UR - https://www.scopus.com/pages/publications/33750146721
M3 - Article
AN - SCOPUS:33750146721
SN - 0146-0404
VL - 37
SP - S782
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 3
ER -