Engineered 3D vascularized neurosphere-derived from induced neural stem cell in an injection-molded microfluidic array

  • Youngtaek Kim
  • , Nari Shin
  • , Jihoon Ko
  • , Jungho Ahn
  • , Kyung Sun Kang
  • , Noo Li Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In vitro platforms for studying the human brain have been developed, and brain organoids using stem cells have been actively studied. However, current organoids are not vascularized and it causes limitation of size and necrosis. Here, we have developed microfluidic array to engineer 3D vascular network with spheroid/organoid using injection molding process. We vascularized neurosphere derived from induced neural stem cells and confirmed structural and functional vessels with microbeads and confocal imaging. This platform can be applied to study the interaction between blood vessels and neurospheres, and ultimately study brain development and disease model.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1518-1519
Number of pages2
ISBN (Electronic)9781733419000
StatePublished - 2019
Externally publishedYes
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 27 Oct 201931 Oct 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period27/10/1931/10/19

Keywords

  • 3D vascular network
  • Induced neural stem cell(iNSC)
  • Microfluidics
  • Neurosphere

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