Effects of O                         2                          and N                         2                         /H                         2                          plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds

Ok Ja Yoon; Hyun Jung Lee; Yeong Mi Jang; Hyun Woo Kim; Won Bok Lee; Sung Su Kim; Nae Eung Lee

doi:10.1016/j.apsusc.2011.05.009

Effects of O ₂ and N ₂ /H ₂ plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds

Ok Ja Yoon
, Hyun Jung Lee
, Yeong Mi Jang
, Hyun Woo Kim
, Won Bok Lee
, Sung Su Kim
, Nae Eung Lee

Department of Advanced Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The O ₂ and N ₂ /H ₂ plasma treatments of single-walled carbon nanotube (SWCNT) papers as scaffolds for enhanced neuronal cell growth were conducted to functionalize their surfaces with different functional groups and to roughen their surfaces. To evaluate the effects of the surface roughness and functionalization modifications of the SWCNT papers, we investigated the neuronal morphology, mitochondrial membrane potential, and acetylcholine/acetylcholinesterase levels of human neuroblastoma during SH-SY5Y cell growth on the treated SWCNT papers. Our results demonstrated that the plasma-chemical functionalization caused changes in the surface charge states with functional groups with negative and positive charges and then the increased surface roughness enhanced neuronal cell adhesion, mitochondrial membrane potential, and the level of neurotransmitter in vitro. The cell adhesion and mitochondrial membrane potential on the negatively charged SWCNT papers were improved more than on the positively charged SWCNT papers. Also, measurements of the neurotransmitter level showed an enhanced acetylcholine level on the negatively charged SWCNT papers compared to the positively charged SWCNT papers.

Original language	English
Pages (from-to)	8535-8541
Number of pages	7
Journal	Applied Surface Science
Volume	257
Issue number	20
DOIs	https://doi.org/10.1016/j.apsusc.2011.05.009
State	Published - 1 Aug 2011

Keywords

Mitochondrial membrane potential
Neuronal activity
Neurotransmitter
Scaffolds
Single-walled carbon nanotube

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.apsusc.2011.05.009

Cite this

Yoon, O. J., Lee, H. J., Jang, Y. M., Kim, H. W., Lee, W. B., Kim, S. S., & Lee, N. E. (2011). Effects of O ₂ and N ₂ /H ₂ plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds Applied Surface Science, 257(20), 8535-8541. https://doi.org/10.1016/j.apsusc.2011.05.009

Yoon, Ok Ja ; Lee, Hyun Jung ; Jang, Yeong Mi et al. / Effects of O ₂ and N ₂ /H ₂ plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds In: Applied Surface Science. 2011 ; Vol. 257, No. 20. pp. 8535-8541.

@article{d371cdb1482d41558f77990ac0f387ce,

title = " Effects of O 2 and N 2 /H 2 plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds ",

abstract = " The O 2 and N 2 /H 2 plasma treatments of single-walled carbon nanotube (SWCNT) papers as scaffolds for enhanced neuronal cell growth were conducted to functionalize their surfaces with different functional groups and to roughen their surfaces. To evaluate the effects of the surface roughness and functionalization modifications of the SWCNT papers, we investigated the neuronal morphology, mitochondrial membrane potential, and acetylcholine/acetylcholinesterase levels of human neuroblastoma during SH-SY5Y cell growth on the treated SWCNT papers. Our results demonstrated that the plasma-chemical functionalization caused changes in the surface charge states with functional groups with negative and positive charges and then the increased surface roughness enhanced neuronal cell adhesion, mitochondrial membrane potential, and the level of neurotransmitter in vitro. The cell adhesion and mitochondrial membrane potential on the negatively charged SWCNT papers were improved more than on the positively charged SWCNT papers. Also, measurements of the neurotransmitter level showed an enhanced acetylcholine level on the negatively charged SWCNT papers compared to the positively charged SWCNT papers.",

keywords = "Mitochondrial membrane potential, Neuronal activity, Neurotransmitter, Scaffolds, Single-walled carbon nanotube",

author = "Yoon, \{Ok Ja\} and Lee, \{Hyun Jung\} and Jang, \{Yeong Mi\} and Kim, \{Hyun Woo\} and Lee, \{Won Bok\} and Kim, \{Sung Su\} and Lee, \{Nae Eung\}",

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Yoon, OJ, Lee, HJ, Jang, YM, Kim, HW, Lee, WB, Kim, SS & Lee, NE 2011, ' Effects of O ₂ and N ₂ /H ₂ plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds ', Applied Surface Science, vol. 257, no. 20, pp. 8535-8541. https://doi.org/10.1016/j.apsusc.2011.05.009

Effects of O ₂ and N ₂ /H ₂ plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds . / Yoon, Ok Ja; Lee, Hyun Jung; Jang, Yeong Mi et al.
In: Applied Surface Science, Vol. 257, No. 20, 01.08.2011, p. 8535-8541.

Research output: Contribution to journal › Article › peer-review

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T1 - Effects of O 2 and N 2 /H 2 plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds

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AU - Lee, Hyun Jung

AU - Jang, Yeong Mi

AU - Kim, Hyun Woo

AU - Lee, Won Bok

AU - Kim, Sung Su

AU - Lee, Nae Eung

PY - 2011/8/1

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N2 - The O 2 and N 2 /H 2 plasma treatments of single-walled carbon nanotube (SWCNT) papers as scaffolds for enhanced neuronal cell growth were conducted to functionalize their surfaces with different functional groups and to roughen their surfaces. To evaluate the effects of the surface roughness and functionalization modifications of the SWCNT papers, we investigated the neuronal morphology, mitochondrial membrane potential, and acetylcholine/acetylcholinesterase levels of human neuroblastoma during SH-SY5Y cell growth on the treated SWCNT papers. Our results demonstrated that the plasma-chemical functionalization caused changes in the surface charge states with functional groups with negative and positive charges and then the increased surface roughness enhanced neuronal cell adhesion, mitochondrial membrane potential, and the level of neurotransmitter in vitro. The cell adhesion and mitochondrial membrane potential on the negatively charged SWCNT papers were improved more than on the positively charged SWCNT papers. Also, measurements of the neurotransmitter level showed an enhanced acetylcholine level on the negatively charged SWCNT papers compared to the positively charged SWCNT papers.

AB - The O 2 and N 2 /H 2 plasma treatments of single-walled carbon nanotube (SWCNT) papers as scaffolds for enhanced neuronal cell growth were conducted to functionalize their surfaces with different functional groups and to roughen their surfaces. To evaluate the effects of the surface roughness and functionalization modifications of the SWCNT papers, we investigated the neuronal morphology, mitochondrial membrane potential, and acetylcholine/acetylcholinesterase levels of human neuroblastoma during SH-SY5Y cell growth on the treated SWCNT papers. Our results demonstrated that the plasma-chemical functionalization caused changes in the surface charge states with functional groups with negative and positive charges and then the increased surface roughness enhanced neuronal cell adhesion, mitochondrial membrane potential, and the level of neurotransmitter in vitro. The cell adhesion and mitochondrial membrane potential on the negatively charged SWCNT papers were improved more than on the positively charged SWCNT papers. Also, measurements of the neurotransmitter level showed an enhanced acetylcholine level on the negatively charged SWCNT papers compared to the positively charged SWCNT papers.

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Yoon OJ, Lee HJ, Jang YM, Kim HW, Lee WB, Kim SS et al. Effects of O ₂ and N ₂ /H ₂ plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds Applied Surface Science. 2011 Aug 1;257(20):8535-8541. doi: 10.1016/j.apsusc.2011.05.009