EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development

Byeong Seong Kim; Hyo Rim Ko; Inwoo Hwang; Sung Woo Cho; Jee Yin Ahn

doi:10.5483/BMBRep.2021.54.8.022

EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development

Byeong Seong Kim, Hyo Rim Ko, Inwoo Hwang, Sung Woo Cho, Jee Yin Ahn

Department of Molecular Cell Biology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.

Original language	English
Pages (from-to)	413-418
Number of pages	6
Journal	BMB Reports
Volume	54
Issue number	8
DOIs	https://doi.org/10.5483/BMBRep.2021.54.8.022
State	Published - 2021

Keywords

ErbB3-binding protein 1
MDM2
Suppressor of variegation 3-9 homolog 1
Ubiquitin-proteasome system

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5483/BMBRep.2021.54.8.022

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title = "EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development",

abstract = "ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.",

keywords = "ErbB3-binding protein 1, MDM2, Suppressor of variegation 3-9 homolog 1, Ubiquitin-proteasome system",

author = "Kim, \{Byeong Seong\} and Ko, \{Hyo Rim\} and Inwoo Hwang and Cho, \{Sung Woo\} and Ahn, \{Jee Yin\}",

year = "2021",

doi = "10.5483/BMBRep.2021.54.8.022",

language = "English",

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T1 - EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development

AU - Kim, Byeong Seong

AU - Ko, Hyo Rim

AU - Hwang, Inwoo

AU - Cho, Sung Woo

AU - Ahn, Jee Yin

PY - 2021

Y1 - 2021

N2 - ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.

AB - ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development.

KW - ErbB3-binding protein 1

KW - MDM2

KW - Suppressor of variegation 3-9 homolog 1

KW - Ubiquitin-proteasome system

UR - https://www.scopus.com/pages/publications/85114310472

U2 - 10.5483/BMBRep.2021.54.8.022

DO - 10.5483/BMBRep.2021.54.8.022

M3 - Article

C2 - 33691908

AN - SCOPUS:85114310472

SN - 1976-6696

VL - 54

SP - 413

EP - 418

JO - BMB Reports

JF - BMB Reports

IS - 8

ER -

EBP1 regulates Suv39H1 stability via the ubiquitin-proteasome system in neural development

Abstract

Keywords

UN SDGs

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