Abstract
The early differentiation of Mycobacterium tuberculosis from non-tuberculous mycobacteria (NTM) and the identification of NTM species are crucial for the proper management of patients with smear-positive sputum. We evaluated the usefulness of a polymerase chain reaction restriction analysis (PRA) method based on the rpoB gene for identifying NTM species in a study of 121 smear-positive respiratory specimens with presumed NTM. The PRA method amplified mycobacterial DNA in 72 specimens (60%) and differentiated NTM species correctly in 68 (94%). The PRA method could be a useful and rapid method for identifying NTM species in smear-positive respiratory specimens when urgent clinical decisions are required.
| Original language | English |
|---|---|
| Pages (from-to) | 1344-1346 |
| Number of pages | 3 |
| Journal | International Journal of Tuberculosis and Lung Disease |
| Volume | 12 |
| Issue number | 11 |
| State | Published - Nov 2008 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Non-tuberculous mycobacteria
- Polymerase chain reaction
- Restriction fragment length polymorphism
- Sputum
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