Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

Beom Soo Shin; Seok Hyun Hong; Hyoung Jun Kim; Hae Seong Yoon; Do Jung Kim; Sang Wook Hwang; Jong Bong Lee; Sun Dong Yoo

doi:10.1365/s10337-008-0871-4

Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

Beom Soo Shin, Seok Hyun Hong, Hyoung Jun Kim, Hae Seong Yoon, Do Jung Kim, Sang Wook Hwang, Jong Bong Lee, Sun Dong Yoo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL^-1 (r = 0.9995), with the limit of quantification at 10 ng mL^-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

Original language	English
Pages (from-to)	295-299
Number of pages	5
Journal	Chromatographia
Volume	69
Issue number	3-4
DOIs	https://doi.org/10.1365/s10337-008-0871-4
State	Published - Feb 2009
Externally published	Yes

Keywords

Column liquid chromatography
Fluorescence detection
Zearalenone and mycotoxin

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10.1365/s10337-008-0871-4

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title = "Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum",

abstract = "A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1\% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL-1 (r = 0.9995), with the limit of quantification at 10 ng mL-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.",

keywords = "Column liquid chromatography, Fluorescence detection, Zearalenone and mycotoxin",

author = "Shin, \{Beom Soo\} and Hong, \{Seok Hyun\} and Kim, \{Hyoung Jun\} and Yoon, \{Hae Seong\} and Kim, \{Do Jung\} and Hwang, \{Sang Wook\} and Lee, \{Jong Bong\} and Yoo, \{Sun Dong\}",

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T1 - Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

AU - Shin, Beom Soo

AU - Hong, Seok Hyun

AU - Kim, Hyoung Jun

AU - Yoon, Hae Seong

AU - Kim, Do Jung

AU - Hwang, Sang Wook

AU - Lee, Jong Bong

AU - Yoo, Sun Dong

PY - 2009/2

Y1 - 2009/2

N2 - A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL-1 (r = 0.9995), with the limit of quantification at 10 ng mL-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

AB - A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL-1 (r = 0.9995), with the limit of quantification at 10 ng mL-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

KW - Column liquid chromatography

KW - Fluorescence detection

KW - Zearalenone and mycotoxin

UR - https://www.scopus.com/pages/publications/59449103762

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DO - 10.1365/s10337-008-0871-4

M3 - Article

AN - SCOPUS:59449103762

SN - 0009-5893

VL - 69

SP - 295

EP - 299

JO - Chromatographia

JF - Chromatographia

IS - 3-4

ER -

Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

Abstract

Keywords

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