Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

Beom Soo Shin; Seok Hyun Hong; Hyoung Jun Kim; Hae Seong Yoon; Do Jung Kim; Sang Wook Hwang; Jong Bong Lee; Sun Dong Yoo

doi:10.1365/s10337-008-0871-4

Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

Beom Soo Shin
, Seok Hyun Hong
, Hyoung Jun Kim
, Hae Seong Yoon
, Do Jung Kim
, Sang Wook Hwang
, Jong Bong Lee
, Sun Dong Yoo

Catholic University of Daegu
Sungkyunkwan University
Natl. Inst. of Toxicological Res.

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL^-1 (r = 0.9995), with the limit of quantification at 10 ng mL^-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

Original language	English
Pages (from-to)	295-299
Number of pages	5
Journal	Chromatographia
Volume	69
Issue number	3-4
DOIs	https://doi.org/10.1365/s10337-008-0871-4
State	Published - Feb 2009
Externally published	Yes

Keywords

Column liquid chromatography
Fluorescence detection
Zearalenone and mycotoxin

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10.1365/s10337-008-0871-4

Cite this

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title = "Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum",

abstract = "A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1\% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL-1 (r = 0.9995), with the limit of quantification at 10 ng mL-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.",

keywords = "Column liquid chromatography, Fluorescence detection, Zearalenone and mycotoxin",

author = "Shin, \{Beom Soo\} and Hong, \{Seok Hyun\} and Kim, \{Hyoung Jun\} and Yoon, \{Hae Seong\} and Kim, \{Do Jung\} and Hwang, \{Sang Wook\} and Lee, \{Jong Bong\} and Yoo, \{Sun Dong\}",

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doi = "10.1365/s10337-008-0871-4",

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T1 - Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

AU - Shin, Beom Soo

AU - Hong, Seok Hyun

AU - Kim, Hyoung Jun

AU - Yoon, Hae Seong

AU - Kim, Do Jung

AU - Hwang, Sang Wook

AU - Lee, Jong Bong

AU - Yoo, Sun Dong

PY - 2009/2

Y1 - 2009/2

N2 - A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL-1 (r = 0.9995), with the limit of quantification at 10 ng mL-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

AB - A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid-liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL-1 (r = 0.9995), with the limit of quantification at 10 ng mL-1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats.

KW - Column liquid chromatography

KW - Fluorescence detection

KW - Zearalenone and mycotoxin

UR - https://www.scopus.com/pages/publications/59449103762

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DO - 10.1365/s10337-008-0871-4

M3 - Article

AN - SCOPUS:59449103762

SN - 0009-5893

VL - 69

SP - 295

EP - 299

JO - Chromatographia

JF - Chromatographia

IS - 3-4

ER -

Development of a sensitive LC assay with fluorescence detection for the determination of zearalenone in rat serum

Abstract

Keywords

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