Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane

Kang Choon Lee; Young Goo Chun; Insoo Kim; Beom Soo Shin; Eun Seok Park; Sun Dong Yoo; Yu Seok Youn

doi:10.1016/j.jchromb.2009.06.010

Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane

Kang Choon Lee
, Young Goo Chun
, Insoo Kim
, Beom Soo Shin
, Eun Seok Park
, Sun Dong Yoo
, Yu Seok Youn

Sungkyunkwan University
Pusan National University
Catholic University of Daegu

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A simple, specific and sensitive derivatization with monobromobimane (mBrB) and the corresponding HPLC-fluorescence quantitation method for the analysis of bucillamine in human plasma was developed and validated. The analytical procedure involves a simple protein precipitation, pre-column fluorescence derivatization, and separation by reversed-phase high performance liquid chromatography (RP-HPLC). The calibration curve showed good linearity over a wide concentration range (50 ng/mL to 10 μg/mL) in human plasma (r² = 0.9998). The lower limit of quantitation (LLOQ) was 50 ng/mL. The average precision and accuracy at LLOQ were within 6.3% and 107.6%, respectively. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (300 mg) of bucillamine to 20 healthy Korean volunteers.

Original language	English
Pages (from-to)	2130-2134
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	22
DOIs	https://doi.org/10.1016/j.jchromb.2009.06.010
State	Published - 15 Jul 2009
Externally published	Yes

Keywords

Bucillamine
Monobromobimane
Pharmacokinetics
Pre-column fluorescence derivatization
RP-HPLC

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10.1016/j.jchromb.2009.06.010

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Lee, K. C., Chun, Y. G., Kim, I., Shin, B. S., Park, E. S., Yoo, S. D., & Youn, Y. S. (2009). Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(22), 2130-2134. https://doi.org/10.1016/j.jchromb.2009.06.010

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title = "Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane",

abstract = "A simple, specific and sensitive derivatization with monobromobimane (mBrB) and the corresponding HPLC-fluorescence quantitation method for the analysis of bucillamine in human plasma was developed and validated. The analytical procedure involves a simple protein precipitation, pre-column fluorescence derivatization, and separation by reversed-phase high performance liquid chromatography (RP-HPLC). The calibration curve showed good linearity over a wide concentration range (50 ng/mL to 10 μg/mL) in human plasma (r2 = 0.9998). The lower limit of quantitation (LLOQ) was 50 ng/mL. The average precision and accuracy at LLOQ were within 6.3\% and 107.6\%, respectively. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (300 mg) of bucillamine to 20 healthy Korean volunteers.",

keywords = "Bucillamine, Monobromobimane, Pharmacokinetics, Pre-column fluorescence derivatization, RP-HPLC",

author = "Lee, \{Kang Choon\} and Chun, \{Young Goo\} and Insoo Kim and Shin, \{Beom Soo\} and Park, \{Eun Seok\} and Yoo, \{Sun Dong\} and Youn, \{Yu Seok\}",

year = "2009",

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doi = "10.1016/j.jchromb.2009.06.010",

language = "English",

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Lee, KC, Chun, YG, Kim, I, Shin, BS, Park, ES, Yoo, SD & Youn, YS 2009, 'Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 22, pp. 2130-2134. https://doi.org/10.1016/j.jchromb.2009.06.010

Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane. / Lee, Kang Choon; Chun, Young Goo; Kim, Insoo et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 22, 15.07.2009, p. 2130-2134.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane

AU - Lee, Kang Choon

AU - Chun, Young Goo

AU - Kim, Insoo

AU - Shin, Beom Soo

AU - Park, Eun Seok

AU - Yoo, Sun Dong

AU - Youn, Yu Seok

PY - 2009/7/15

Y1 - 2009/7/15

N2 - A simple, specific and sensitive derivatization with monobromobimane (mBrB) and the corresponding HPLC-fluorescence quantitation method for the analysis of bucillamine in human plasma was developed and validated. The analytical procedure involves a simple protein precipitation, pre-column fluorescence derivatization, and separation by reversed-phase high performance liquid chromatography (RP-HPLC). The calibration curve showed good linearity over a wide concentration range (50 ng/mL to 10 μg/mL) in human plasma (r2 = 0.9998). The lower limit of quantitation (LLOQ) was 50 ng/mL. The average precision and accuracy at LLOQ were within 6.3% and 107.6%, respectively. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (300 mg) of bucillamine to 20 healthy Korean volunteers.

AB - A simple, specific and sensitive derivatization with monobromobimane (mBrB) and the corresponding HPLC-fluorescence quantitation method for the analysis of bucillamine in human plasma was developed and validated. The analytical procedure involves a simple protein precipitation, pre-column fluorescence derivatization, and separation by reversed-phase high performance liquid chromatography (RP-HPLC). The calibration curve showed good linearity over a wide concentration range (50 ng/mL to 10 μg/mL) in human plasma (r2 = 0.9998). The lower limit of quantitation (LLOQ) was 50 ng/mL. The average precision and accuracy at LLOQ were within 6.3% and 107.6%, respectively. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (300 mg) of bucillamine to 20 healthy Korean volunteers.

KW - Bucillamine

KW - Monobromobimane

KW - Pharmacokinetics

KW - Pre-column fluorescence derivatization

KW - RP-HPLC

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

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ER -

Lee KC, Chun YG, Kim I, Shin BS, Park ES, Yoo SD et al. Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 Jul 15;877(22):2130-2134. doi: 10.1016/j.jchromb.2009.06.010

Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane

Abstract

Keywords

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