Deoxypodophyllotoxin inhibits the expression of intercellular adhesion molecule-1 induced by tumor necrosis factor-α in murine lung epithelial cells

Meihua Jin; Eunkyung Lee; Ju Hye Yang; Yue Lu; Sang Gu Kang; Young Chae Chang; Seung Ho Lee; Seok Jong Suh; Cheorl Ho Kim; Hyeun Wook Chang

doi:10.1248/bpb.33.1

Deoxypodophyllotoxin inhibits the expression of intercellular adhesion molecule-1 induced by tumor necrosis factor-α in murine lung epithelial cells

Meihua Jin, Eunkyung Lee, Ju Hye Yang, Yue Lu, Sang Gu Kang, Young Chae Chang, Seung Ho Lee, Seok Jong Suh, Cheorl Ho Kim, Hyeun Wook Chang

Department of Biological Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Intercellular adhesion molecule-1 (ICAM-1) is associated with processes of inflammation. We investigated the effects of deoxypodophyllotoxin (DPT) on tumor necrosis factor-α (TNF-α) induced ICAM-1 expression in the mouse lung epithelial cell line, LA4. DPT (5 to 20 nM) inhibited TNF-α-induced ICAM-1 expression through nuclear factor-kappa B (NF-κB) in a dose-dependent manner and repressed ICAM-1 promoter activity. NF-κB reporter gene activity and DNA binding activity were also strongly inhibited. In addition, DPT inhibited degradation by the TNF-α induced inhibitory kB-α (IκB-α) in a concentration-dependent manner. Taken together with our previous results suggest DPT might provide a basis for novel anti-inflammatory drug development.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Biological and Pharmaceutical Bulletin
Volume	33
Issue number	1
DOIs	https://doi.org/10.1248/bpb.33.1
State	Published - Jan 2010

Keywords

Deoxypodophyllotoxin
Intercellular adhesion molecule-1
Lipopolysaccharide
Nuclear factor-kappa B
Tumor necrosis factor-α

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1248/bpb.33.1

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@article{a2ef3b9826ad4cc8a918d4525f976ff8,

title = "Deoxypodophyllotoxin inhibits the expression of intercellular adhesion molecule-1 induced by tumor necrosis factor-α in murine lung epithelial cells",

abstract = "Intercellular adhesion molecule-1 (ICAM-1) is associated with processes of inflammation. We investigated the effects of deoxypodophyllotoxin (DPT) on tumor necrosis factor-α (TNF-α) induced ICAM-1 expression in the mouse lung epithelial cell line, LA4. DPT (5 to 20 nM) inhibited TNF-α-induced ICAM-1 expression through nuclear factor-kappa B (NF-κB) in a dose-dependent manner and repressed ICAM-1 promoter activity. NF-κB reporter gene activity and DNA binding activity were also strongly inhibited. In addition, DPT inhibited degradation by the TNF-α induced inhibitory kB-α (IκB-α) in a concentration-dependent manner. Taken together with our previous results suggest DPT might provide a basis for novel anti-inflammatory drug development.",

keywords = "Deoxypodophyllotoxin, Intercellular adhesion molecule-1, Lipopolysaccharide, Nuclear factor-kappa B, Tumor necrosis factor-α",

author = "Meihua Jin and Eunkyung Lee and Yang, \{Ju Hye\} and Yue Lu and Kang, \{Sang Gu\} and Chang, \{Young Chae\} and Lee, \{Seung Ho\} and Suh, \{Seok Jong\} and Kim, \{Cheorl Ho\} and Chang, \{Hyeun Wook\}",

year = "2010",

month = jan,

doi = "10.1248/bpb.33.1",

language = "English",

volume = "33",

pages = "1--5",

journal = "Biological and Pharmaceutical Bulletin",

issn = "0918-6158",

publisher = "Pharmaceutical Society of Japan",

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TY - JOUR

T1 - Deoxypodophyllotoxin inhibits the expression of intercellular adhesion molecule-1 induced by tumor necrosis factor-α in murine lung epithelial cells

AU - Jin, Meihua

AU - Lee, Eunkyung

AU - Yang, Ju Hye

AU - Lu, Yue

AU - Kang, Sang Gu

AU - Chang, Young Chae

AU - Lee, Seung Ho

AU - Suh, Seok Jong

AU - Kim, Cheorl Ho

AU - Chang, Hyeun Wook

PY - 2010/1

Y1 - 2010/1

N2 - Intercellular adhesion molecule-1 (ICAM-1) is associated with processes of inflammation. We investigated the effects of deoxypodophyllotoxin (DPT) on tumor necrosis factor-α (TNF-α) induced ICAM-1 expression in the mouse lung epithelial cell line, LA4. DPT (5 to 20 nM) inhibited TNF-α-induced ICAM-1 expression through nuclear factor-kappa B (NF-κB) in a dose-dependent manner and repressed ICAM-1 promoter activity. NF-κB reporter gene activity and DNA binding activity were also strongly inhibited. In addition, DPT inhibited degradation by the TNF-α induced inhibitory kB-α (IκB-α) in a concentration-dependent manner. Taken together with our previous results suggest DPT might provide a basis for novel anti-inflammatory drug development.

AB - Intercellular adhesion molecule-1 (ICAM-1) is associated with processes of inflammation. We investigated the effects of deoxypodophyllotoxin (DPT) on tumor necrosis factor-α (TNF-α) induced ICAM-1 expression in the mouse lung epithelial cell line, LA4. DPT (5 to 20 nM) inhibited TNF-α-induced ICAM-1 expression through nuclear factor-kappa B (NF-κB) in a dose-dependent manner and repressed ICAM-1 promoter activity. NF-κB reporter gene activity and DNA binding activity were also strongly inhibited. In addition, DPT inhibited degradation by the TNF-α induced inhibitory kB-α (IκB-α) in a concentration-dependent manner. Taken together with our previous results suggest DPT might provide a basis for novel anti-inflammatory drug development.

KW - Deoxypodophyllotoxin

KW - Intercellular adhesion molecule-1

KW - Lipopolysaccharide

KW - Nuclear factor-kappa B

KW - Tumor necrosis factor-α

UR - https://www.scopus.com/pages/publications/75149194393

U2 - 10.1248/bpb.33.1

DO - 10.1248/bpb.33.1

M3 - Article

C2 - 20045926

AN - SCOPUS:75149194393

SN - 0918-6158

VL - 33

SP - 1

EP - 5

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

IS - 1

ER -

Deoxypodophyllotoxin inhibits the expression of intercellular adhesion molecule-1 induced by tumor necrosis factor-α in murine lung epithelial cells

Abstract

Keywords

UN SDGs

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