Deoxynivalenol induces apoptosis and disrupts cellular homeostasis through MAPK signaling pathways in bovine mammary epithelial cells

Deoxynivalenol (DON), a fungus-derived mycotoxin, also known as vomitoxin, is found in a wide range of cereal grains and grain-based food products. The biological toxicity of DON has been described in various species, but its toxicity and functional effects in mammary epithelial cells are unclear. In this study, we investigated the effect of DON on bovine mammary epithelial (MAC-T) cells using mechanistic approaches. We detected DON-induced cell cycle abrogation and calcium deficiency, leading to apoptotic cell death via MAPK signaling pathways. Moreover, we studied the transcriptional activation of blood and milk junctional regulators as well as inflammatory cytokines in response to DON. The results of this study contribute to a comprehensive understanding of DON-associated toxicity mechanisms in bovine mammary epithelial cells, which may facilitate the enhancement of milk stabilization in parallel with the establishment of safety profiles to protect against DON contamination in livestock farms and in the food industry. Deoxynivalenol contamination induces cell cycle arrest and apoptosis in bovine mammary epithelial cells via MAPK signaling pathways.