TY - JOUR
T1 - Cytotoxic constituents of Amanita subjunquillea
AU - Kim, Ki Hyun
AU - Choi, Sang Un
AU - Park, Ki Moon
AU - Seok, Soon Ja
AU - Lee, Kang Ro
PY - 2008/5
Y1 - 2008/5
N2 - As part of our systematic study of Korean toxic mushrooms, we have investigated the constituents of Amanita subjunquillea. The column chromatographic separation of the MeOH extract of A. subjunquillea led to the isolation of four ergosterols, two cerebrosides and four cyclopeptides. Their structures were determined by spectroscopic methods to be (22E,24R)-5α, 8α-epidioxyergosta-6,9,22-triene-3β-ol (1), (22E,24R)-5α, 8α-epidioxyergosta-6,22-dien-3β-ol (2), (22E,24R)-5α,6α- epoxyergosta-8,22-diene-3β,7β-diol (3), (24S)-ergost-7-en-3β-ol (4), 8,9-dihydrosoyacerebroside I (5), soyacerebroside I (6), β-amanitin (7), phalloin (8), α-amanitin (9), and phalloidin (10). The compounds 1-6 and 8 were isolated for the first time from this mushroom. The isolated compounds were evaluated for the cytotoxicity against A549, SK-OV-3, SK-MEL-2 and HCT15 cells. Compound 9 exhibited significant cytotoxic activity against A549, SK-OV-3, SK-MEL-2 and HCT15 with ED50 values of 1.47, 0.26, 1.57 and 1.32 μM, respectively.
AB - As part of our systematic study of Korean toxic mushrooms, we have investigated the constituents of Amanita subjunquillea. The column chromatographic separation of the MeOH extract of A. subjunquillea led to the isolation of four ergosterols, two cerebrosides and four cyclopeptides. Their structures were determined by spectroscopic methods to be (22E,24R)-5α, 8α-epidioxyergosta-6,9,22-triene-3β-ol (1), (22E,24R)-5α, 8α-epidioxyergosta-6,22-dien-3β-ol (2), (22E,24R)-5α,6α- epoxyergosta-8,22-diene-3β,7β-diol (3), (24S)-ergost-7-en-3β-ol (4), 8,9-dihydrosoyacerebroside I (5), soyacerebroside I (6), β-amanitin (7), phalloin (8), α-amanitin (9), and phalloidin (10). The compounds 1-6 and 8 were isolated for the first time from this mushroom. The isolated compounds were evaluated for the cytotoxicity against A549, SK-OV-3, SK-MEL-2 and HCT15 cells. Compound 9 exhibited significant cytotoxic activity against A549, SK-OV-3, SK-MEL-2 and HCT15 with ED50 values of 1.47, 0.26, 1.57 and 1.32 μM, respectively.
KW - Amanita subjunquillea
KW - Cyclopeptide
KW - Cytotoxicity
KW - Ergosterol
UR - https://www.scopus.com/pages/publications/43749103136
U2 - 10.1007/s12272-001-1196-3
DO - 10.1007/s12272-001-1196-3
M3 - Article
C2 - 18481012
AN - SCOPUS:43749103136
SN - 0253-6269
VL - 31
SP - 579
EP - 586
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
IS - 5
ER -